Manoharan Ranjith Kumar, Lee Jin-Hyung, Kim Yong-Guy, Kim Soon-Il, Lee Jintae
a School of Chemical Engineering , Yeungnam University , Gyeongsan , Republic of Korea.
b Nareso Research Center , Suwon , Republic of Korea.
Biofouling. 2017 Feb;33(2):143-155. doi: 10.1080/08927014.2017.1280731. Epub 2017 Feb 3.
Candida albicans is one of the most common fungal pathogens, and causes systemic and invasive infections in humans. C. albicans biofilms are composed of yeast and hyphal and pseudohyphal elements, and the transition of yeast to the hyphal stage could be a virulence factor. In this study, diverse essential oils were initially investigated for anti-biofilm activity against C. albicans strains, and cascarilla bark oil and helichrysum oil and their components α-longipinene (a major constituent of both) and linalool were found to markedly inhibit biofilm formation without affecting planktonic cell growth. Moreover, α-longipinene and linalool were found to synergistically reduce biofilm formation. Notably, treatments with cascarilla bark oil, helichrysum oil, α-longipinene, or linalool clearly inhibited hyphal formation, and this appeared to be largely responsible for their anti-biofilm effect. Furthermore, the two essential oils, α-longipinene and linalool, reduced C. albicans virulence in Caenorhabditis elegans.
白色念珠菌是最常见的真菌病原体之一，可引起人类的全身性和侵袭性感染。白色念珠菌生物膜由酵母、菌丝和假菌丝成分组成，酵母向菌丝阶段的转变可能是一种毒力因子。在本研究中，首先对多种精油针对白色念珠菌菌株的抗生物膜活性进行了研究，发现巴豆树皮油和蜡菊油及其成分α-长叶蒎烯（两者的主要成分）和芳樟醇可显著抑制生物膜形成，而不影响浮游细胞生长。此外，发现α-长叶蒎烯和芳樟醇可协同减少生物膜形成。值得注意的是，用巴豆树皮油、蜡菊油、α-长叶蒎烯或芳樟醇处理可明显抑制菌丝形成，这似乎在很大程度上是它们抗生物膜作用的原因。此外，α-长叶蒎烯和芳樟醇这两种精油降低了白色念珠菌在秀丽隐杆线虫中的毒力。
