Hsu Hsin-Yen, Chen Bing-Huei
Department of Food Science, Fu Jen Catholic University, New Taipei City 24205, Taiwan.
Department of Nutrition, China Medical University, Taichung 40402, Taiwan.
Pharmaceutics. 2022 May 2;14(5):980. doi: 10.3390/pharmaceutics14050980.
The objectives of this study were to determine carotenoid composition in sweet potato (TNG66) peel and prepare carotenoid nanoemulsion to study its inhibition effect on breast cancer cells MCF-7 and tumors in mice. Results showed that a total of 10 carotenoids were separated within 30 min by employing a YMC C30 column and a gradient mobile phase of methanol/acetonitrile/water (74:14:12, ) and dichloromethane (B) with a flow rate of 1 mL/min, column temperature of 25 °C, and detection wavelength of 450 nm. Following quantitation, all-trans-β-carotene was present in the highest amount (663.8 μg/g). The method validation data demonstrated a high accuracy and precision of this method. The carotenoid nanoemulsion was prepared by mixing an appropriate proportion of carotenoid extract, Tween 80, PEG 400, soybean oil and deionized water with the mean particle size being 15.7 nm (transmission electron microscope (TEM)), polydispersity index 0.238, encapsulation efficiency 97% and zeta potential -69.8 mV. A high stability of carotenoid nanoemulsion was shown over a 90-day storage period at 25 °C and during heating at 100 °C for 2 h. The release percentage of total carotenoids from carotenoid nanoemulsion under gastric and intestinal condition was 18.3% and 49.1%, respectively. An antiproliferation study revealed that carotenoid nanoemulsion was more effective than carotenoid extract in inhibiting the growth of human breast cancer cells MCF-7. Following treatments of paclitaxel (10 μg/mL), carotenoid nanoemulsion (20 and 10 μg/mL) and carotenoid extract (20 and 10 μg/mL), the tumor weight of mice respectively decreased by 77.4, 56.2, 40.3, 36.1 and 18.7%, as well as tumor volume of mice by 75.4, 65.0, 49.7, 46.7 and 26.5%. Also, both carotenoid extract and nanoemulsion could reduce the levels of epidermal growth factor (EGF) and (vascular endothelial growth factor (VEGF) in serum, with the latter being more effective. This finding suggested that carotenoid nanoemulsion was more effective than carotenoid extract in inhibiting tumor growth in mice.
本研究的目的是测定甘薯（台农66号）皮中的类胡萝卜素组成，并制备类胡萝卜素纳米乳剂，以研究其对乳腺癌细胞MCF-7和小鼠肿瘤的抑制作用。结果表明，采用YMC C30柱，以甲醇/乙腈/水（74:14:12）和二氯甲烷（B）为梯度流动相，流速为1 mL/min，柱温为25℃，检测波长为450 nm，在30分钟内共分离出10种类胡萝卜素。定量分析后发现，全反式β-胡萝卜素含量最高（663.8μg/g）。方法验证数据表明该方法具有较高的准确度和精密度。通过将适量比例的类胡萝卜素提取物、吐温80、聚乙二醇400、大豆油和去离子水混合制备类胡萝卜素纳米乳剂，其平均粒径为15.7nm（透射电子显微镜（TEM）），多分散指数为0.238，包封率为97%，ζ电位为-69.8mV。在25℃下储存90天以及在100℃加热2小时期间，类胡萝卜素纳米乳剂均表现出较高的稳定性。在胃和肠道条件下，类胡萝卜素纳米乳剂中总类胡萝卜素的释放率分别为18.3%和49.1%。一项抗增殖研究表明，类胡萝卜素纳米乳剂在抑制人乳腺癌细胞MCF-7生长方面比类胡萝卜素提取物更有效。在分别用紫杉醇（10μg/mL）、类胡萝卜素纳米乳剂（20和10μg/mL）和类胡萝卜素提取物（20和10μg/mL）处理后，小鼠的肿瘤重量分别降低了77.4%、56.2%、40.3%、36.1%和18.7%，小鼠的肿瘤体积分别降低了75.4%、65.0%、49.7%、46.7%和26.5%。此外，类胡萝卜素提取物和纳米乳剂均可降低血清中表皮生长因子（EGF）和血管内皮生长因子（VEGF）的水平，其中纳米乳剂的效果更显著。这一发现表明，类胡萝卜素纳米乳剂在抑制小鼠肿瘤生长方面比类胡萝卜素提取物更有效。
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