Serramito María, Pereira-da-Mota Ana F, Carpena-Torres Carlos, Huete-Toral Fernando, Alvarez-Lorenzo Carmen, Carracedo Gonzalo
Ocupharm Research Group, Department of Optometry and Vision, Faculty of Optics and Optometry, Complutense University of Madrid, C/Arcos de Jalón 118, 28037 Madrid, Spain.
I+D Farma Group (GI-1645), Departamento de Farmacología, Farmacia y Tecnología Farmacéutica, Facultad de Farmacia, Instituto de Materiales (iMATUS), Health Research Institute of Santiago de Compostela (IDIS), Universidade de Santiago de Compostela, 15782 Santiago de Compostela, Spain.
Pharmaceutics. 2022 May 9;14(5):1019. doi: 10.3390/pharmaceutics14051019.
(1) Background: The purpose of this study was to synthesize melatonin-eluting contact lenses (CLs) and evaluate both the ocular kinetics of the released melatonin and its effect on tear volume and intraocular pressure. (2) Methods: In vitro, melatonin-eluting CLs were synthesized by using non-functionalized (HEMA) and functionalized (HEMA/APMA) monomers. In vivo, a short-term prospective and randomized study was performed on 15 rabbits divided into two groups: 12 rabbits wearing functionalized CLs and 3 rabbits without CLs as a control. The melatonin levels in tears, aqueous humor, vitreous body and retina, tear volume, and intraocular pressure were measured for 8 h. (3) Results: In vitro, both monomers did not show differences in terms of melatonin loading and release (p ≥ 0.05). In vivo, the melatonin concentration was elevated in tears and aqueous humor after 2 and 4 h of wearing CLs, respectively (p < 0.05). Additionally, the CLs increased tear volume for 2 h (p < 0.05). (4) Conclusions: The melatonin-eluting CLs released their content over the ocular surface for at least 2 h, which was associated with a secretagogue effect on tear volume. However, the increased amount of melatonin found in the aqueous humor had no effect on intraocular pressure.
(1) 背景：本研究的目的是合成褪黑素缓释隐形眼镜（CLs），并评估释放的褪黑素的眼部动力学及其对泪液量和眼压的影响。(2) 方法：在体外，使用未功能化的（HEMA）和功能化的（HEMA/APMA）单体合成褪黑素缓释隐形眼镜。在体内，对15只兔子进行了一项短期前瞻性随机研究，分为两组：12只佩戴功能化隐形眼镜的兔子和3只不佩戴隐形眼镜作为对照的兔子。测量泪液、房水、玻璃体和视网膜中的褪黑素水平、泪液量和眼压，持续8小时。(3) 结果：在体外，两种单体在褪黑素负载和释放方面均未显示出差异（p≥0.05）。在体内，佩戴隐形眼镜2小时和4小时后，泪液和房水中的褪黑素浓度分别升高（p<0.05）。此外，隐形眼镜使泪液量增加了2小时（p<0.05）。(4) 结论：褪黑素缓释隐形眼镜在眼表释放其成分至少2小时，这与对泪液量的促分泌作用有关。然而，在房水中发现的褪黑素增加量对眼压没有影响。
