Shirosaki Yuki, Tsukatani Yasuyo, Okamoto Kohei, Hayakawa Satoshi, Osaka Akiyoshi
Faculty of Engineering, Kyushu Institute of Technology, 1-1 Sensuicho, Tobata-ku, Kitakyushu 804-8550, Japan.
Graduate School of Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.
Pharmaceutics. 2022 May 23;14(5):1111. doi: 10.3390/pharmaceutics14051111.
Chitosan is a cationic polymer that forms polymerized membranes upon reaction with anionic polymers. Chitosan-carboxymethyl cellulose (CMC) capsules are drug delivery carrier candidates whose mechanical strength and permeability must be controlled to achieve sustained release. In this study, the capsules were prepared from chitosan-γ-glycidoxypropyltrimethoxysilane (GPTMS)-CMC. The mechanical stability of the capsules was improved by crosslinking the chitosan with GPTMS. The capsules were then coated with hydroxyapatite (HAp) by alternately soaking them in calcium chloride solution and disodium hydrogen phosphate solution to prevent rapid initial drug release. Cytochrome C (CC), as a model drug, was introduced into the capsules via two routes, impregnation and injection, and then the CC released from the capsules was examined. HAp was found to be deposited on the internal and external surfaces of the capsules. The amount of CC introduced, and the release rate were reduced by the HAp coating. The injection method was found to result in the greatest CC loading.
壳聚糖是一种阳离子聚合物，与阴离子聚合物反应时会形成聚合膜。壳聚糖-羧甲基纤维素（CMC）胶囊是药物递送载体候选物，其机械强度和渗透性必须加以控制以实现缓释。在本研究中，胶囊由壳聚糖-γ-缩水甘油基丙基三甲氧基硅烷（GPTMS）-CMC制备。通过壳聚糖与GPTMS交联提高了胶囊的机械稳定性。然后将胶囊交替浸泡在氯化钙溶液和磷酸氢二钠溶液中，用羟基磷灰石（HAp）包衣，以防止药物快速初始释放。作为模型药物的细胞色素C（CC）通过浸渍和注射两种途径引入胶囊，然后检测从胶囊中释放的CC。发现HAp沉积在胶囊的内表面和外表面。HAp包衣降低了CC的引入量和释放速率。发现注射法导致的CC载药量最大。
