通过形成非经典分子间结构实现自发DNA联会

Severov Viacheslav, Tsvetkov Vladimir, Barinov Nikolay, Babenko Vladislav, Klinov Dmitry, Pozmogova Galina

Federal Research and Clinical Center of Physical-Chemical Medicine, Malaya Pirogovskaya Str. 1a, 119435 Moscow, Russia.

Institute of Biodesign and Complex System Modeling, I.M. Sechenov First Moscow State Medical University, Trubetskaya Str. 8-2, 119991 Moscow, Russia.

Polymers (Basel). 2022 May 23;14(10):2118. doi: 10.3390/polym14102118.

We report the spontaneous formation of DNA-DNA junctions in solution in the absence of proteins visualised using atomic force microscopy. The synapsis position fits with potential G-quadruplex (G4) sites. In contrast to the Holliday structure, these conjugates have an affinity for G4 antibodies. Molecular modelling was used to elucidate the possible G4/IM-synaptic complex structures. Our results indicate a new role of the intermolecular noncanonical structures in chromatin architecture and genomic rearrangement.

我们报告了在无蛋白质溶液中自发形成的DNA-DNA连接，采用原子力显微镜观察到。联会位置与潜在的G-四链体（G4）位点相符。与霍利迪结构不同，这些共轭物对G4抗体具有亲和力。使用分子建模来阐明可能的G4/IM-突触复合体结构。我们的结果表明分子间非经典结构在染色质结构和基因组重排中具有新作用。