Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China.
Laboratory of Fish Molecular Immunology, College of Fisheries and Life Science, Shanghai Ocean University, Shanghai, China; Laboratory of Marine Biology and Biotechnology, Qingdao National Laboratory for Marine Science and Technology, Qingdao, China.
Fish Shellfish Immunol. 2023 Feb;133:108561. doi: 10.1016/j.fsi.2023.108561. Epub 2023 Jan 20.
In recent years, more and more researchers have devoted to the study of circular RNAs (circRNAs) in noncoding RNAs. As an important regulator in a variety of biological processes, circRNAs are relatively abundant in the study of mammals, while research in lower vertebrates is still lacking. In this study, we found a circRNA, circPlce1, related to innate immune response in Miichthys miiuy (miiuy croaker). The experimental results confirmed that circPlce1 could promote the production of antiviral genes and inflammatory response under the stimulation of poly (I: C) and LPS. We also confirmed that circPlce1 can promote NF-κB and IRF3 pathways through luciferase reporter assay experiment. In addition, we also found that circPlce1 can promote cell proliferation and improve cell viability. In conclusion, our results showed that circPlce1 plays an active role in regulating inflammatory response, cell proliferation and cell viability, providing a foundation for the study of the biological function of circRNAs in the innate immune response in teleost fish.
近年来，越来越多的研究人员致力于研究非编码 RNA 中的环状 RNA（circRNAs）。作为各种生物过程中的重要调节剂，circRNAs 在哺乳动物的研究中相对丰富，而在较低等脊椎动物中的研究仍然缺乏。在这项研究中，我们在 Miichthys miiuy（米氏黄鱼）中发现了一个与先天免疫反应相关的环状 RNA，circPlce1。实验结果证实，circPlce1 可以在 poly（I:C）和 LPS 的刺激下促进抗病毒基因和炎症反应的产生。我们还通过荧光素酶报告基因实验证实了 circPlce1 可以通过促进 NF-κB 和 IRF3 通路来发挥作用。此外，我们还发现 circPlce1 可以促进细胞增殖并提高细胞活力。总之，我们的结果表明 circPlce1 在调节炎症反应、细胞增殖和细胞活力方面发挥着积极作用，为研究 circRNAs 在硬骨鱼先天免疫反应中的生物学功能提供了基础。
