Bacteriology Laboratory, Department of Infectious and Parasitic Diseases, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Food Science Department, FARAH and Faculty of Veterinary Medicine, University of Liège, 4000 Liège, Belgium.
Viruses. 2022 May 17;14(5):1079. doi: 10.3390/v14051079.
Antibiotic resistance represents a major public health concern requiring new alternatives including phage therapy. belongs to the ESKAPE bacteria and can cause urinary tract infections (UTIs). The aims of this study were to isolate and characterize new bacteriophages against a strain isolated from UTIs and to assess their efficacy in vitro and in vivo in a larvae model. For this purpose, two bacteriophages were newly isolated against an ST13 strain isolated from a UTI and identified as K3 capsular types by gene PCR. Genomic analysis showed that these bacteriophages, named vB_KpnP_K3-ULINTkp1 and vB_KpnP_K3-ULINTkp2, belong to the genus. Bacteriophage vB_KpnP_K3-ULINTkp1 had the narrowest host spectrum (targeting only K3), while vB_KpnP_K3-ULINTkp2 also infected other types. Short adsorption times and latent periods were observed for both bacteriophages. In vivo experiments showed their ability to replicate in larvae and to decrease host bacterial titers. Moreover, both bacteriophages improved the survival of the infected larvae. In conclusion, these two bacteriophages had different in vitro properties and showed in vivo efficacy in a model with a better efficiency for vB_KpnP_K3-ULINTkp2.
抗生素耐药性是一个主要的公共卫生关注点，需要新的替代品，包括噬菌体疗法。属于 ESKAPE 细菌，可引起尿路感染（UTIs）。本研究的目的是分离和鉴定针对从 UTIs 分离的 株的新型噬菌体，并在 幼虫模型中评估其体外和体内的疗效。为此，针对从 UTI 中分离出的一株 ST13 株，新分离出两种噬菌体，并通过 基因 PCR 将其鉴定为 K3 荚膜型。基因组分析表明，这些命名为 vB_KpnP_K3-ULINTkp1 和 vB_KpnP_K3-ULINTkp2 的噬菌体属于 属。噬菌体 vB_KpnP_K3-ULINTkp1 的宿主谱最窄（仅靶向 K3），而 vB_KpnP_K3-ULINTkp2 还感染其他 型。两种噬菌体均表现出较短的吸附时间和潜伏期。体内实验表明它们能够在 幼虫中复制并降低宿主细菌滴度。此外，两种噬菌体都提高了感染幼虫的存活率。总之，这两种噬菌体具有不同的体外特性，并在 模型中显示出体内疗效，vB_KpnP_K3-ULINTkp2 的效率更高。
