Bacteriophage Laboratory, Department of Phage Therapy, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wrocław, Poland.
Laboratory of Phage Molecular Biology, Department of Phage Therapy, Hirszfeld Institute of Immunology and Experimental Therapy, Polish Academy of Sciences, 53-114 Wrocław, Poland.
Viruses. 2022 Jul 12;14(7):1518. doi: 10.3390/v14071518.
In an era of antibiotic therapy crisis caused by spreading antimicrobial resistance, and when recurrent urinary tract infections constitute a serious social and medical problem, the isolation and complex characterization of phages with a potential therapeutic application represents a promising solution. It is an inevitable, and even a necessary direction in the development of current phage research. In this paper, we present two newly isolated myoviruses that show lytic activity against multidrug-resistant clinical isolates of spp. (, , and ), the genomes of which belong to a poorly represented phage group. Both phages were classified as part of the subfamily (Entb_43 was recognized as and Entb_45 as ). Phage lytic spectra ranging from 40 to 60% were obtained. The most effective phage-to-bacteria ratios (MOI = 0.01 and MOI = 0.001) for both the phage amplification and their lytic activity against planktonic bacteria were also estimated. Complete adsorption to host cells were obtained after about 20 min for Entb_43 and 10 min for Entb_45. The phage lysates retained their initial titers even during six months of storage at both -70 °C and 4 °C, whereas storage at 37 °C caused a complete loss in their activity. We showed that phages retained their activity after incubation with solutions of silver and copper nanoparticles, which may indicate possible synergistic antibacterial activity. Moreover, a significant reduction in phage titers was observed after incubation with a disinfectant containing octenidinum dihydrochloridum and phenoxyethanol, as well as with 70% ethanol. The observed maintenance of phage activity during incubation in a urine sample, along with other described properties, may suggest a therapeutic potential of phages at the infection site after intravesical administration.
在抗生素治疗危机时代，由于抗菌药物耐药性的传播，复发性尿路感染构成了严重的社会和医学问题，具有潜在治疗应用的噬菌体的分离和复杂特征分析代表了一种有前途的解决方案。这是当前噬菌体研究发展的必然，甚至是必要方向。在本文中，我们介绍了两种新分离的肌尾噬菌体，它们对多种耐药性临床分离株表现出裂解活性，这些分离株的基因组属于一个代表性较差的噬菌体群。两种噬菌体均被归类为 亚科（Entb_43 被识别为 和 Entb_45 为 ）。获得了范围从 40%到 60%的噬菌体裂解谱。还估计了两种噬菌体的最有效噬菌体与细菌的比值（MOI = 0.01 和 MOI = 0.001），用于噬菌体扩增及其对浮游细菌的裂解活性。Entb_43 约 20 分钟，Entb_45 约 10 分钟即可完成对宿主细胞的完全吸附。噬菌体裂解液即使在 -70°C 和 4°C 下储存六个月，其初始滴度也保持不变，而在 37°C 下储存则会完全丧失其活性。我们表明，噬菌体在与银和铜纳米粒子溶液孵育后保留了其活性，这可能表明存在协同抗菌活性。此外，在与含有奥替尼啶二盐酸盐和苯氧乙醇的消毒剂以及 70%乙醇孵育后，观察到噬菌体滴度显著降低。在尿样中孵育时观察到噬菌体活性的维持，以及其他描述的特性，可能表明在膀胱内给药后，噬菌体在感染部位具有治疗潜力。
