监测、交叉抗性、遗传和拟除虫菊酯类杀虫剂吡丙醚对褐飞虱Nilaparvata lugens 在中国的适合度代价。

Monitoring, cross-resistance, inheritance, and fitness costs of brown planthoppers, Nilaparvata lugens, resistance to pymetrozine in China.

机构信息

College of Plant Protection, Nanjing Agricultural University, Nanjing, China.

出版信息

Pest Manag Sci. 2022 Oct;78(10):3980-3987. doi: 10.1002/ps.7017. Epub 2022 Jun 17.

DOI:10.1002/ps.7017

PMID:35633099

Abstract

BACKGROUND

The brown planthopper, Nilaparvata lugens, is considered the most destructive pest of rice in many Asian countries including China. Use of pymetrozine in insect resistance management (IRM) has been one strategy to control this pest. In this study, we reported the status of pymetrozine resistance in Nilaparvata lugens (Stål) collected from China over the period 2017-2021 and selected a strain of N. lugens resistant to pymetrozine and evaluated the cross-resistance, inheritance and fitness costs of the resistance.

RESULTS

Monitoring data (2017-2021) showed that field populations of N. lugens in China developed moderate- to high-level pymetrozine resistance during these 5 years. By continuous selection with pymetrozine in the lab, the pymetrozine selected N. lugens strain (Pym-R ) developed a 225.2-fold resistance compared to a susceptible strain. The Pym-R strain showed high cross-resistance to dinotefuran (66.6-fold) and low cross-resistance to nitenpyram (5.2-fold) and sulfoxaflor (5.8-fold). Inheritance pattern analysis of Pym-R revealed that resistance to pymetrozine was polygenic, autosomal and incompletely dominant. Fitness costs of pymetrozine resistance were present in Pym-R and WA2020 strains with a relative fitness of 0.72 and 0.60, respectively. The developmental duration of Pym-R and WA2020 was significantly longer and hatchability was significantly lower compared to pymetrozine-susceptible strain (Pym-S).

CONCLUSIONS

N. lugens has developed high level of resistance to pymetrozine. Pymetrozine-resistance brown planthopper had cross-resistance with some of neonicotinoids such as dinotefuran, nitenpyram and sulfoxaflor. The autosomal, incompletely dominant and polygenic resistance to pymetrozine in N. lugens and the fitness costs associated with this resistance can be exploited in IRM strategies to preserve the lifetime of pymetrozine for control of N. lugens in China. © 2022 Society of Chemical Industry.

摘要

背景

褐飞虱，Nilaparvata lugens，被认为是包括中国在内的许多亚洲国家水稻最具破坏性的害虫。在昆虫抗药性管理（IRM）中使用吡丙醚是控制这种害虫的一种策略。在本研究中，我们报告了 2017-2021 年期间中国采集的褐飞虱对吡丙醚的抗药性现状，并选择了对吡丙醚具有抗药性的褐飞虱品系，并评估了抗药性的交互抗性、遗传和适合度代价。

结果

监测数据（2017-2021 年）显示，在这 5 年期间，中国田间种群的褐飞虱对吡丙醚产生了中到高水平的抗药性。通过在实验室中连续用吡丙醚选择，吡丙醚选择的褐飞虱品系（Pym-R）与敏感品系相比，抗性提高了 225.2 倍。Pym-R 品系对啶虫脒表现出高交互抗性（66.6 倍），对吡虫啉（5.2 倍）和噻虫氟（5.8 倍）表现出低交互抗性。Pym-R 的遗传模式分析表明，对吡丙醚的抗性是多基因的、常染色体的和不完全显性的。Pym-R 和 WA2020 品系的吡丙醚抗性存在适合度代价，相对适合度分别为 0.72 和 0.60。与吡丙醚敏感品系（Pym-S）相比，Pym-R 和 WA2020 的发育时间明显延长，孵化率明显降低。