Department of Microbiology, Nepal Medical College and Teaching Hospital, Jorpati, Attarkhel, Kathmandu Nepal.
JNMA J Nepal Med Assoc. 2022 Apr 15;60(248):384-388. doi: 10.31729/jnma.7219.
Lower respiratory tract infection accounts for a great burden of disease worldwide. The problem has further increased due to increasing antimicrobial resistance. This study was done to find out prevalence of positive bacterial culture among lower respiratory tract specimens of patients in a tertiary care centre.
A descriptive cross-sectional study was done in the Laboratory of Clinical Microbiology in a tertiary care centre from May, 2021 to October, 2021. Ethical approval was received from the Institutional Review Committee (Reference number: 045-077/078). A total of 635 specimens were collected by convenience sampling. The specimens were cultured as per standard microbiological techniques. Antibiotic susceptibility was performed following Clinical and Laboratory Standards Institute (2020) guidelines. Microsoft Excel was used for data entry and analysis. Point estimate at 95% Confidence Interval was calculated along with frequency and proportion.
Among the 635 lower respiratory specimens, 112 (17.63%) (111.97 to 112.03 at 95% Confidence Interval) showed positive bacterial culture. Klebsiella pneumoniae 44 (37.93%) was the commonest isolate followed by Acinetobacter calcoaceticus baumannii complex 34 (29.31%).
The prevalence of positive bacterial culture among lower respiratory specimens was lower when compared to other studies done in similar settings.
antibiotic resistance; gram negative bacteria; respiratory tract infections.
下呼吸道感染在全球造成了巨大的疾病负担。由于抗菌药物耐药性的不断增加，这一问题进一步加剧。本研究旨在调查三级护理中心患者下呼吸道标本中阳性细菌培养的发生率。
2021 年 5 月至 2021 年 10 月，在三级护理中心的临床微生物学实验室进行了一项描述性横断面研究。本研究已获得机构审查委员会的伦理批准（参考编号：045-077/078）。通过方便抽样收集了 635 份标本。根据标准微生物技术对标本进行培养。按照临床和实验室标准协会（2020 年）的指南进行抗生素敏感性检测。使用 Microsoft Excel 进行数据录入和分析。计算了 95%置信区间的点估计值，以及频率和比例。
在 635 份下呼吸道标本中，有 112 份（17.63%）（95%置信区间为 111.97%至 112.03%）显示阳性细菌培养。肺炎克雷伯菌 44 株（37.93%）是最常见的分离株，其次是鲍曼不动杆菌复合群 34 株（29.31%）。
与类似环境下进行的其他研究相比，下呼吸道标本中阳性细菌培养的发生率较低。
抗生素耐药性；革兰氏阴性菌；呼吸道感染。
