Zuo Kangjia, Li Huanan, Chen Jianhui, Ran Qiuping, Huang Mengtian, Cui Xinxin, He Lili, Liu Jiashu, Jiang Zhengbing
State Key Laboratory of Biocatalysis and Enzyme Engineering, Hubei University, Wuhan, China.
Hubei Key Laboratory of Industrial Biotechnology, School of Life Sciences, Hubei University, Wuhan, China.
Front Microbiol. 2022 May 11;13:901690. doi: 10.3389/fmicb.2022.901690. eCollection 2022.
Biotransformation has gained increasing attention due to its being an eco-friendly way for the production of value-added chemicals. The present study aimed to assess the potential of ZB1 on guaiacyl lignin monomers biotransformation for the production of vanillin. Consequently, isoeugenol, eugenol, and vanillyl alcohol could be transformed into vanillin by ZB1. Based on the structural alteration of masson pine and the increase of total phenol content in the supernatant, ZB1 exhibited potential in lignin depolymerization and valorization using masson pine as the substrate. As the precursors of vanillin, 61.1% of isoeugenol and eugenol in pyrolyzed bio-oil derived from masson pine could be transformed into vanillin by ZB1. Four monooxygenases with high specific activity were identified that were involved in the transformation process. Thus, ZB1 could emerge as a candidate in the biosynthesis of vanillin by using wide guaiacyl precursors as the substrates.
生物转化作为一种生产高附加值化学品的环保方式，已越来越受到关注。本研究旨在评估ZB1对愈创木基木质素单体进行生物转化以生产香草醛的潜力。结果表明，异丁香酚、丁香酚和香草醇可被ZB1转化为香草醛。基于马尾松的结构变化以及上清液中总酚含量的增加，ZB1在以马尾松为底物的木质素解聚和增值方面显示出潜力。作为香草醛的前体，马尾松热解生物油中61.1%的异丁香酚和丁香酚可被ZB1转化为香草醛。鉴定出四种具有高比活性的单加氧酶参与了转化过程。因此，ZB1有望成为以广泛的愈创木基前体为底物生物合成香草醛的候选者。
