Engineering Research Centre of Biomass Conversion and Pollution Prevention Control of Anhui Provincial Department of Education, Fuyang 236037, PR China; School of Chemistry and Materials Engineering, Fuyang Normal University, Fuyang 236037, PR China.
Engineering Research Centre of Biomass Conversion and Pollution Prevention Control of Anhui Provincial Department of Education, Fuyang 236037, PR China.
Bioresour Technol. 2020 Aug;310:123445. doi: 10.1016/j.biortech.2020.123445. Epub 2020 Apr 24.
Using tobacco straw (Ts) and lignin as the sole carbon source, a strain was isolated from Ts and identified as Bacillus amyloliquefaciens SL-7 by 16S rDNA gene-sequencing technology.7-day incubation of Bacillus amyloliquefaciens SL-7 can reduce the chemical oxygen demand (COD) by 69.35% in lignin mineral salt medium. The activity of Manganese peroxidase (MnP) reached maximum level 258.57 U L, and Lignin peroxidase (Lip) was 422.68 U L at 4th day. The highest Laccase (Lac) activity (55.95 U L) was observed at 3th day. After straw-liquid fermentation degradation of 15 days, the bacterial could degrade 28.55% lignin of the straw which was close to that of fungi. Compared with the control group and effective microorganisms (EM) group, the lignin degradation rate in Bacillus amyloliquefaciens SL-7 group increased by 22.26% and 11.70% at 41-day compost fermentation of tobacco straw. These show the strain has strong lignin degradation performance.
利用烟秆（Ts）和木质素作为唯一碳源，从烟秆中分离出一株菌，并通过 16S rDNA 基因测序技术鉴定为解淀粉芽孢杆菌 SL-7。7 天培养解淀粉芽孢杆菌 SL-7 可使木质素无机盐培养基中的化学需氧量（COD）降低 69.35%。锰过氧化物酶（MnP）活性在第 4 天达到最大水平 258.57 U L，木质素过氧化物酶（Lip）为 422.68 U L。漆酶（Lac）活性最高（55.95 U L）出现在第 3 天。经过 15 天的秸秆液体发酵降解，细菌可降解 28.55%的秸秆木质素，接近真菌的降解率。与对照组和有效微生物（EM）组相比，在 41 天的烟草秸秆堆肥发酵中，解淀粉芽孢杆菌 SL-7 组的木质素降解率分别提高了 22.26%和 11.70%。这些表明该菌株具有较强的木质素降解性能。
