Department of Biochemistry and Biophysics, Stockholm University, 10691 Stockholm, Sweden.
Department of Medical Biochemistry and Cell Biology, University of Gothenburg, 41390 Gothenburg, Sweden.
STAR Protoc. 2022 May 25;3(2):101359. doi: 10.1016/j.xpro.2022.101359. eCollection 2022 Jun 17.
Many aspects of mitochondrial gene expression are still unknown, which can be attributed to limitations in molecular tools. Here, we present a protocol to introduce reporter genes into the mitochondrial genome of budding yeast, . Mitochondrially encoded reporter constructs can be used to interrogate various aspects of mitochondrial gene expression. The power of this technique is exemplified by a mitochondrially encoded nanoluciferase, which allows to monitor levels of mitochondrial translation under a variety of growth conditions.
线粒体基因表达的许多方面仍然未知，这可以归因于分子工具的局限性。在这里，我们提出了一种将报告基因引入芽殖酵母线粒体基因组的方案，。线粒体编码的报告基因构建体可用于研究线粒体基因表达的各个方面。这项技术的强大功能通过一个线粒体编码的纳米荧光素酶来说明，它可以在各种生长条件下监测线粒体翻译的水平。
