Reproductive Center, Zaozhuang Maternal and Child Health Care Hospital, Zaozhuang, China.
Beijing Key Laboratory of Animal Genetic Improvement, National Engineering Laboratory for Animal Breeding, Key Laboratory of Animal Genetics and Breeding of the Ministry of Agriculture, College of Animal Science and Technology, China Agricultural University, Beijing, China.
Front Endocrinol (Lausanne). 2022 May 13;13:853999. doi: 10.3389/fendo.2022.853999. eCollection 2022.
In this study, two experiments were performed to assess the effect and the role of melatonin on human embryo quality.
Experiment I: A total of 42 repeated-poor-quality-embryo patients were enrolled, with a total of 181 oocytes retrieval cycles. After IVF, for the same patient, the MT cycles group (10 M melatonin added to the culture medium; n=48) were compared with the previous non-MT cycles group (n=133), following by culture to blastocyst stage and embryo transfer. 31 patients were transplanted with 65 embryo transfer, including 24 MT embryo transfer, 41 non-MT embryo transfer. Cycle outcomes were compared between the two groups. Experiment II:A total of 143 supernumerary human cleavage-stage embryos (from non-repeated-poor-quality-embryo patients) vitrified on Day 3 after IVF were warmed and randomized into two groups: melatonin group (10 M melatonin added to the culture medium; n=71) and control group (n=72), and then cultured for 72 h. Rate of blastocyst and high-quality blastocyst, reactive oxygen species (ROS) levels of culture media as well as embryonic , , , , , gene expression levels were analyzed.
Experiment I: Results showed that the rate of Day 3 high-quality embryos (29.6% 19.5%) in the MT cycles group was significantly higher than that in the non-MT cycles group (<0.05). The rate of available blastocysts (17.1% 12.7%) and clinical pregnancy rate (25.0% 17.1%) were in tendency higher in the group treated with melatonin (>0.05). Experiment II:Results showed that the blastocyst rates in the melatonin administered group were significantly higher than in control group (42.25% 26.38%, <0.05). There were no significant differences in high-quality blastocyst rates. In addition, quantitative PCR showed that the expression of was significantly upregulated by melatonin treatment (<0.05), while there were no significant differences in the expression of , , , and gene as well as the levels of ROS.
These data showed that melatonin supplement in the culture medium will improve Day 3 high-quality embryos rate of repeated-poor-quality-embryo patients and improve blastocyst rate of vitrified-warmed cleavage-stage embryos, suggesting that melatonin intervention may provide a potential rescue strategy for IVF failures.
identifier [ChiCTR2200059773].
本研究进行了两项实验，旨在评估褪黑素对人类胚胎质量的作用和影响。
实验 I：共纳入 42 例重复出现胚胎质量差的患者，共进行了 181 个卵母细胞采集周期。在体外受精（IVF）后，对于同一患者，将 MT 周期组（在培养物中添加 10 μM 褪黑素；n=48）与之前的非-MT 周期组（n=133）进行比较，然后培养至囊胚阶段并进行胚胎移植。31 例患者共移植 65 个胚胎转移，其中 MT 胚胎转移 24 个，非-MT 胚胎转移 41 个。比较两组之间的周期结局。实验 II：共对 143 个来自非重复胚胎质量差的患者的超数人类卵裂期胚胎（在 IVF 后第 3 天）进行玻璃化，并进行解冻和随机分为两组：褪黑素组（在培养物中添加 10 μM 褪黑素；n=71）和对照组（n=72），然后培养 72 小时。分析囊胚率和高质量囊胚率、培养物中的活性氧（ROS）水平以及胚胎、、、、、、 基因的表达水平。
实验 I：结果显示，MT 周期组第 3 天的高质量胚胎率（29.6% 19.5%）明显高于非-MT 周期组（<0.05）。在接受褪黑素治疗的组中，可利用囊胚率（17.1% 12.7%）和临床妊娠率（25.0% 17.1%）呈上升趋势（>0.05）。实验 II：结果显示，褪黑素处理组的囊胚率明显高于对照组（42.25% 26.38%，<0.05）。高质量囊胚率无显著差异。此外，定量 PCR 显示，褪黑素处理后 基因的表达明显上调（<0.05），而 、、、 基因的表达以及 ROS 水平无显著差异。
这些数据表明，在培养基中添加褪黑素可提高重复出现胚胎质量差的患者第 3 天的高质量胚胎率，并提高玻璃化解冻的卵裂期胚胎的囊胚率，表明褪黑素干预可能为 IVF 失败提供潜在的补救策略。
标识符 [ChiCTR2200059773]。
