Department of Ophthalmology, The First People's Hospital of Zunyi, The Third Affiliated Hospital of Zunyi Medical University, Zunyi, Guizhou, China.
Bioengineered. 2022 May;13(5):13293-13299. doi: 10.1080/21655979.2022.2080367.
T helper 17 (Th17) cells regulate inflammatory processes and are implicated in pathogenesis of proliferative diabetic retinopathy (PDR) through modulation of interleukin-17 (IL-17). IL-35, anti-inflammatory factor, negatively mediates IL-17 expression and Th17 differentiation. In this study, the role of IL-35 in PDR was assessed. The results showed that IL-35 was down-regulated, while IL-17 was up-regulated, in peripheral blood mononuclear cells (PBMCs) of PDR patients. In addition, immunofluorescence analysis indicated that frequency of Th17 cells was enhanced in the PBMCs of PDR patients. However, incubation with IL-35 reduced the Th17 cell frequency and decreased the level of IL-17 in CD4 T lymphocytes. Moreover, the levels of transcription factors essential for Th17 differentiation, ROR α (retinoid-related orphan receptor alpha) and ROR γt, were reduced by IL-35 treatment. In conclusion, IL-35 reduced level of IL-17 and inhibited Th17 differentiation to protect against PDR.
辅助性 T 细胞 17（Th17）细胞调节炎症过程，并通过调节白细胞介素-17（IL-17）参与增殖性糖尿病视网膜病变（PDR）的发病机制。抗炎因子 IL-35 通过负向调节 IL-17 的表达和 Th17 分化来发挥作用。本研究评估了 IL-35 在 PDR 中的作用。结果表明，PDR 患者外周血单个核细胞（PBMCs）中 IL-35 下调，而 IL-17 上调。此外，免疫荧光分析表明，PDR 患者 PBMCs 中的 Th17 细胞频率增加。然而，IL-35 孵育可降低 Th17 细胞频率，并降低 CD4 T 淋巴细胞中 IL-17 的水平。此外，IL-35 处理可降低 Th17 分化所必需的转录因子 RORα（维甲酸相关孤儿受体α）和 RORγt 的水平。综上所述，IL-35 降低了 IL-17 的水平，并抑制了 Th17 分化，从而起到了预防 PDR 的作用。
