胶带粘贴法细胞学检查在犬皮肤马拉色菌定量检测中的重复性和可再现性
Repeatability and reproducibility of microscopic examination of adhesive tape strip cytology slides for the quantification of Malassezia spp. in canine skin.
机构信息
Clinic of Medicine, Faculty of Veterinary Science, University of Thessaly, Karditsa, Greece.
Veterinary Clinic, Army Veterinary Training and Health Center, Stratigou N. Plastira, Larisa, Greece.
出版信息
Vet Dermatol. 2022 Aug;33(4):305-e71. doi: 10.1111/vde.13076. Epub 2022 May 30.
BACKGROUND
The optimal microscopic magnification and number of optical fields of adhesive tape strip cytological slides that should be examined when searching for Malassezia yeasts on canine skin are unknown.
OBJECTIVES
To determine the optimal magnification and the minimum number of optical fields that should be examined to maximise intraobserver repeatability and interobserver reproducibility.
MATERIALS AND METHODS
Seven experienced examiners counted, twice, the number of yeasts in 10, 20, 30, 40 and 50 optical fields of 40 slides at ×400 and ×1000 magnification.
RESULTS
The number of yeasts per unit surface area was significantly higher at ×1000 compared to ×400 magnification. Repeatability and reproducibility for counting the yeasts was very poor.
CONCLUSIONS AND CLINICAL RELEVANCE
Adhesive tape strip cytological slides should be examined microscopically for Malassezia spp. at ×1000 magnification. The repeatability of this examination for counting the yeasts is poor.
背景
在犬皮肤寻找马拉色菌时，最佳的显微镜放大倍数和胶带条细胞学载玻片的光学视野数量尚不清楚。
目的
确定最佳放大倍数和应检查的最小光学视野数量，以最大限度地提高观察者内重复性和观察者间可重复性。
材料和方法
7 名有经验的检查者在×400 和×1000 放大倍数下，对 40 张载玻片的 10、20、30、40 和 50 个光学视野中的酵母数量进行了两次计数。
结果
与×400 放大倍数相比，单位面积的酵母数量在×1000 放大倍数下显著更高。计数酵母的重复性和再现性非常差。
结论和临床相关性
应在×1000 放大倍数下用显微镜检查胶带条细胞学载玻片以查找马拉色菌。该检查计数酵母的重复性较差。
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