Department of Oncology and Metabolism, University of Sheffield, Sheffield, UK.
University of Bayreuth, Experimental Physics 1, Bayreuth, Germany.
Methods Mol Biol. 2022;2476:171-181. doi: 10.1007/978-1-0716-2221-6_13.
Atomic force microscopy is an ideal tool to map topography and mechanical properties of materials on the micro- and nanoscale. Here, we describe its application to measure and analyze the mechanics, in particular the effective Young's elastic modulus E of the mammalian nucleus in live cells. We present three approaches which enable the mechanics to be probed under varying conditions. This includes fully adhered cells, initially adhered cells which lack an established cytoskeleton, and purified nuclei to study their isolated response.
原子力显微镜是一种理想的工具，可用于绘制微纳尺度材料的形貌和机械性能图。在这里，我们描述了它在测量和分析力学，特别是哺乳动物细胞核的有效杨氏弹性模量 E 方面的应用，这些力学特性在活细胞中是可以被探测到的。我们提出了三种方法，可以在不同的条件下进行力学探测。这包括完全附着的细胞、最初附着但缺乏已建立的细胞骨架的细胞，以及纯化的核，以研究它们的分离反应。
