Department of Microbiology and Immunology, Institute of Biomedicine, Sahlgrenska Academy, University of Gothenburg, Gothenburg, Sweden.
Departments of Physics and Biology, University of York, York, UK.
Methods Mol Biol. 2022;2476:183-190. doi: 10.1007/978-1-0716-2221-6_14.
Proteins are one of the key components of cellular life that play a crucial role in most biological processes. Therefore, quantification of protein copy numbers is essential for revealing and better understanding of cellular behavior and functions. Here we describe a single-molecule fluorescence-based method of protein copy number quantification directly in living cells. This enables quick and reliable estimations and comparison of the protein of interest abundance without implementing large-scale studies.
蛋白质是细胞生命的关键组成部分之一，在大多数生物过程中发挥着至关重要的作用。因此，蛋白质拷贝数的定量对于揭示和更好地理解细胞行为和功能至关重要。在这里，我们描述了一种在活细胞中直接进行蛋白质拷贝数定量的单分子荧光法。这使得无需进行大规模研究即可快速、可靠地估计和比较感兴趣的蛋白质的丰度。
