Prairie Diagnostic Services Inc., Saskatoon, Saskatchewan, Canada.
Department of Veterinary Pathology, Western College of Veterinary Medicine, University of Saskatchewan, Saskatoon, Saskatchewan, Canada.
Vet Med Sci. 2022 Sep;8(5):1969-1974. doi: 10.1002/vms3.851. Epub 2022 May 30.
Porcine circovirus 3 (PCV3) has been detected in pigs worldwide and associated with several clinical signs.
To investigate the genetic diversity of PCV3 strains circulating in Canada, 44 PCV3 positive samples from Saskatchewan (2/44), Manitoba (2/44), Quebec (4/44), Alberta (11/44) and Ontario (25/44) submitted to diagnostic laboratories in Canada between 2019 and 2021 were sequenced and analyzed.
Phylogenetic analysis of capsid genes showed that all of the 44 Canadian strains classified into PCV3a and segregated into seven lineages with common amino acid changes observed at A24V, R27K, N56D, T77S, Q98R, L150I (F) and R168K positions.
Future studies are required to determine whether the polymorphisms in capsid proteins, as revealed in this study, could be associated with differences in the pathogenicity or antigenicity of PCV3 strains. This is the first phylogenetic analysis of PCV3 strains among different provinces in Canada.
猪圆环病毒 3 型（PCV3）已在全球范围内的猪群中被检测到，并与多种临床症状相关。
为了研究在加拿大流行的 PCV3 毒株的遗传多样性，对 2019 年至 2021 年间提交给加拿大诊断实验室的来自萨斯喀彻温省（2/44）、马尼托巴省（2/44）、魁北克省（4/44）、艾伯塔省（11/44）和安大略省（25/44）的 44 份 PCV3 阳性样本进行了测序和分析。
对衣壳基因的系统进化分析表明，所有 44 株加拿大分离株均归属于 PCV3a 型，并分为 7 个谱系，在 A24V、R27K、N56D、T77S、Q98R、L150I（F）和 R168K 位置观察到常见的氨基酸变化。
未来需要研究是否衣壳蛋白的多态性与 PCV3 毒株的致病性或抗原性的差异有关。这是对加拿大不同省份的 PCV3 毒株进行的首次系统进化分析。
