Dinh Phat Xuan, Nguyen Hai Ngoc, Lai Danh Cong, Nguyen Toan Tat, Nguyen Nam Minh, Do Duy Tien
Faculty of Biological Sciences, Nong Lam University-HCMC, Thu Duc City, Vietnam.
Department of Infectious Disease and Veterinary Public Health, Faculty of Animal Husbandry and Veterinary Medicine, Nong Lam University-HCMC, Quarter 6, Linh Trung ward, Thu Duc City, Vietnam.
Arch Virol. 2023 Jan 4;168(1):30. doi: 10.1007/s00705-022-05661-x.
Porcine circovirus type 3 (PCV3) was first detected in 2016 and has been reported in many pig-producing countries around the world, including Vietnam. PCV3 has been found in complex cases with multiple clinical syndromes in swine. In this study, we investigated the genetic diversity of PCV3 strains circulating in Vietnam. A total of 249 samples were collected from swine farms located in eight provinces of Vietnam, and 11.65% (29/249) of these samples were found to contain PCV3. The ORF2 genes from the 29 PCV3-positive samples were amplified, purified, and sequenced. Phylogenetic analysis showed that 23 of these strains belonged to the PCV3b subtype, while the remaining six strains belonged to subtype c and subtype a (a-1 and a-2). Analysis of the ORF2 genes indicated that the 29 PCV3 strains had high sequence identity (96.90-100% at the genomic level and 96.19-100% at the amino acid level). Fifteen amino acid substitutions were found in predicted B-cell epitopes in the capsid proteins of the Vietnamese PCV3 strains.
猪圆环病毒3型（PCV3）于2016年首次被检测到，并且在包括越南在内的世界许多养猪国家都有报道。PCV3已在猪的多种临床综合征复杂病例中被发现。在本研究中，我们调查了越南流行的PCV3毒株的遗传多样性。从越南八个省份的猪场共采集了249份样本，其中11.65%（29/249）的样本被发现含有PCV3。对29份PCV3阳性样本的ORF2基因进行了扩增、纯化和测序。系统发育分析表明，这些毒株中有23株属于PCV3b亚型，其余6株属于c亚型和a亚型（a-1和a-2）。对ORF2基因的分析表明，这29株PCV3毒株具有较高的序列同一性（基因组水平为96.90-100%，氨基酸水平为96.19-100%）。在越南PCV3毒株衣壳蛋白预测的B细胞表位中发现了15个氨基酸替换。
