Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 66003, China.
Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao, 66003, China; Laboratory for Marine Fisheries Science and Food Production Processes, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 266237, China.
Fish Shellfish Immunol. 2022 Jul;126:211-216. doi: 10.1016/j.fsi.2022.05.051. Epub 2022 May 27.
Crassostrea nippona is a kind of oysters with great development value as it can be edible in summer for its late reproductive period. Salinity is one of the important limiting abiotic factors to the survival and distribution of this stenohaline species. To better understand the physiological and immunological response of C. nippona to varying environmental salinities, the effects of low salinity on the hemolymph osmolality and gill transcriptome were investigated in this study. The osmolality of hemolymph in vivo and surrounding water were assessed regularly over one week at five test salinities ranging from 5 psμ to 30 psμ. They reached osmotic equilibrium within hours above 15 psμ but remained hyperosmotic at 10 and 5 psμ for the whole sampling period. Through comparative transcriptome analysis, there were less differentially expressed genes (DEGs) in pairwise comparison of S1 (10 psμ) vs S3 (30 psμ) than in S2 (20 psμ) vs S3. KEGG enrichment analysis identified ubiquitin-mediated proteolysis and mitochondrial apoptosis pathway specifically enriched at 10 psμ. This study gained comprehensive insights on the low salinity response of C. nippona at the molecular level, which provide a theoretical basis for understanding the immune mechanism under low salinity stress.
日本真牡蛎是一种具有巨大发展价值的牡蛎，因其繁殖期较晚，夏季可食用。盐度是限制该狭盐种生存和分布的重要非生物因素之一。为了更好地了解日本真牡蛎对不同环境盐度的生理和免疫反应，本研究探讨了低盐度对其血淋巴渗透压和鳃转录组的影响。在五个测试盐度（5 psμ 至 30 psμ）下，每周定期评估体内血淋巴渗透压和周围水的渗透压。在 15 psμ 以上的盐度下，它们在数小时内达到渗透平衡，但在整个采样期间，在 10 和 5 psμ 仍保持高渗状态。通过比较转录组分析，在 S1（10 psμ）与 S3（30 psμ）的两两比较中，差异表达基因（DEGs）比在 S2（20 psμ）与 S3 的比较中少。KEGG 富集分析鉴定出泛素介导的蛋白水解和线粒体凋亡途径在 10 psμ 时特异性富集。本研究从分子水平上全面了解了日本真牡蛎对低盐度的反应，为理解低盐度胁迫下的免疫机制提供了理论依据。
