Institute of Molecular and Clinical Ophthalmology Basel, Basel, Switzerland.
Department of Ophthalmology, University of Basel, Basel, Switzerland.
Nat Biotechnol. 2022 Oct;40(10):1447-1451. doi: 10.1038/s41587-022-01312-3. Epub 2022 May 30.
We present FLASH-seq (FS), a full-length single-cell RNA sequencing (scRNA-seq) method with increased sensitivity and reduced hands-on time compared to Smart-seq3. The entire FS protocol can be performed in ~4.5 hours, is simple to automate and can be easily miniaturized to decrease resource consumption. The FS protocol can also use unique molecular identifiers (UMIs) for molecule counting while displaying reduced strand-invasion artifacts. FS will be especially useful for characterizing gene expression at high resolution across multiple samples.
我们提出了 FLASH-seq（FS）方法，与 Smart-seq3 相比，该方法具有更高的灵敏度和更低的人工操作时间，可实现全长单细胞 RNA 测序（scRNA-seq）。整个 FS 方案可在~4.5 小时内完成，易于自动化，并可轻松微型化以减少资源消耗。FS 方案还可以使用独特的分子标识符（UMIs）进行分子计数，同时显示减少的链入侵伪影。FS 将特别有助于在多个样本中以高分辨率描述基因表达。
