Key Lab for Special Functional Materials of the Ministry of Education, and School of Materials, Henan University, Kaifeng, 475004, China.
Nanoscale. 2023 Mar 23;15(12):5560-5578. doi: 10.1039/d2nr07247e.
Fluorescence immunoassays have been given considerable attention among the quantitative detection methods in the clinical medicine and food safety testing fields. In particular, semiconductor quantum dots (QDs) have become ideal fluorescent probes for highly sensitive and multiplexed detection due to their unique photophysical properties, and the QD fluorescence-linked immunosorbent assay (FLISA) with high sensitivity, high accuracy, and high throughput has been greatly developed recently. In this manuscript, the advantages of applying QDs to FLISA platforms and some strategies for their application to diagnostics and food safety are discussed. Given the rapid development of this field, we classify these strategies based on the combination of QD types and detection targets, including traditional QDs or QD micro/nano-spheres-FLISA, and multiple FLISA platforms. In addition, some new sensors based on the QD-FLISA are introduced; this is one of the hot spots in this field. The current focus and future direction of QD-FLISA are also discussed, which provides important guidance for the further development of FLISA.
荧光免疫分析在临床医学和食品安全检测领域的定量检测方法中受到了相当大的关注。特别是，由于半导体量子点 (QD) 具有独特的光物理性质，因此成为高灵敏度和多重检测的理想荧光探针，而具有高灵敏度、高精度和高通量的 QD 荧光免疫分析 (FLISA) 最近得到了极大的发展。在本文中，讨论了将 QD 应用于 FLISA 平台的优势，以及将其应用于诊断和食品安全的一些策略。鉴于该领域的快速发展，我们根据 QD 类型和检测靶标组合对这些策略进行分类，包括传统 QD 或 QD 微/纳球-FLISA，以及多种 FLISA 平台。此外，还介绍了一些基于 QD-FLISA 的新型传感器；这是该领域的热点之一。还讨论了 QD-FLISA 的当前焦点和未来方向，这为进一步发展 FLISA 提供了重要指导。
