Tasopoulos Theodoros, Vrioni Georgia, Naka Olga, Diamantatou Theodora, Zoidis Panagiotis, Tsakris Athanasios
Department of Microbiology, Medical School, National and Kapodistrian University of Athens, Athens, Greece.
Department of Prosthodontics, School of Dentistry, Aristotle University of Thessaloniki, Thessaloniki, Greece.
J Prosthodont. 2023 Apr;32(4):292-297. doi: 10.1111/jopr.13551. Epub 2022 Jun 16.
Knowledge about quantifying the number as well as the retention and adhesion of Candida albicans blastoconidia to silicone denture liners is limited. Thus, the aim of this in vitro study was to explore the adherence of C. albicans to the surface of five long-term silicone-based soft denture lining materials, using artificial saliva.
A total of 50 specimens (10 × 10 × 3 mm) of five long-term resilient liners (Molloplast-B; GC Reline Soft; Elite Soft Relining; Tokuyama Sofreliner S; Ufigel SC), bonded to a computer-aided design and computer-aided manufacturing denture base, were prepared. The specimens were inoculated and incubated in artificial saliva for 1 and 24 hours with a standardized (2.8 × 10 cfu/ml) C. albicans suspension. At the end of the incubation period, the specimens were stained with acridine orange and observed using fluorescence microscopy.
After 1 hour and in 24 hours, Molloplast B demonstrated significantly earlier adherence of C. albicans cells compared to the other chairside materials (p < 0.001 and p < 0.001, respectively), where the mean number of cells also increased in the frontal parts. Regarding the rate of C. albicans proliferation from 1 to 24 hours within the materials, there was an increase in all materials (Molloplast B: p < 0.001; GC Reline Soft: p = 0.220; Elite Soft Relining: p = 0.032; Tokuyama Sofreliner S: p = 0.001; Ufigel Sc: p = 0.001). The Ufigel Sc showed a significant 2.5-fold increase at 24 hours.
Long-term silicone denture liners accumulate a significant amount of C. albicans blastoconidia and their coverage by them increases progressively over time.
关于白色念珠菌芽生孢子数量的量化以及其在硅橡胶义齿衬垫上的留存和黏附的相关知识有限。因此，本体外研究的目的是使用人工唾液来探究白色念珠菌在五种长期使用的硅基软质义齿衬垫材料表面的黏附情况。
制备了50个样本（10×10×3毫米），这些样本来自五种长期弹性衬垫（Molloplast - B；GC Reline Soft；Elite Soft Relining；Tokuyama Sofreliner S；Ufigel SC），并与计算机辅助设计和计算机辅助制造的义齿基托相连。将样本接种于人工唾液中，使用标准化的（2.8×10 cfu/ml）白色念珠菌悬液孵育1小时和24小时。在孵育期结束时，样本用吖啶橙染色并使用荧光显微镜观察。
1小时和24小时后，与其他椅旁材料相比，Molloplast B显示白色念珠菌细胞的黏附明显更早（分别为p < 0.001和p < 0.001），其中前部的细胞平均数也增加。关于材料中白色念珠菌从1小时到24小时的增殖率，所有材料均有增加（Molloplast B：p < 0.001；GC Reline Soft：p = 0.220；Elite Soft Relining：p = 0.032；Tokuyama Sofreliner S：p = 0.001；Ufigel Sc：p = 0.001）。Ufigel Sc在24小时时显示出显著的2.5倍增长。
长期使用的硅橡胶义齿衬垫会积累大量白色念珠菌芽生孢子，且随着时间推移，它们被白色念珠菌的覆盖程度会逐渐增加。
