Bhatta Dharm Raj, Koirala Sumnima, Baral Abha, Amatya Niroj Man, Parajuli Sulochana, Shrestha Rajani, Hamal Deependra, Nayak Niranjan, Gokhale Shishir
Department of Microbiology, Manipal College of Medical Sciences, Pokhara, Nepal.
Department of Medical Microbiology, Nobel College, Kathmandu, Nepal.
Can J Infect Dis Med Microbiol. 2022 May 21;2022:1023241. doi: 10.1155/2022/1023241. eCollection 2022.
Bacterial contamination in intensive care units is an important risk factor associated with increasing incidences of nosocomial infections. This study was conducted to study the bacterial colonization on commonly touched objects of intensive care units and antibiotic resistance pattern of bacterial isolates.
This study was conducted in different intensive care units of Manipal Teaching Hospital, Pokhara, Nepal. A total of 235 swabs were collected from surfaces of bed rails, monitors, door handles, IV stands, telephone sets, nursing stations, medicine trolleys, sphygmomanometers, wash basin taps, dressing drums, stethoscopes, pulse oximeters, ventilators, defibrillators, and stretchers. Isolation, identification, and antibiotic susceptibility tests of the bacteria were performed following standard microbiological techniques.
Of 235 samples, bacterial growth was observed in 152 samples. A total of 90 samples of were isolated from 235 samples. Most of the sampling sites included in this study were found contaminated with . . The highest number of . was cultured from the surface of bed rails. Of the total . isolates, 54.4% (49/90) were methicillin-resistant (MRSA). Vancomycin resistance was detected among 8.1% MRSA isolates (4/49). species were the commonest Gram-negative bacterial isolate.
Bacterial contamination of the objects/instruments of the ICU was recorded to be high. The most common contaminating bacteria were . with a high percentage of MRSA and emergence of VRSA. Periodic microbiological surveillance, detection of contaminated sites, and effective decontamination methods would minimize the colonization by potential pathogens and their transmission.
重症监护病房中的细菌污染是与医院感染发病率增加相关的重要危险因素。本研究旨在调查重症监护病房中常见接触物体上的细菌定植情况以及细菌分离株的抗生素耐药模式。
本研究在尼泊尔博卡拉马尼帕尔教学医院的不同重症监护病房进行。共从床栏、监护仪、门把手、静脉输液架、电话机、护士站、药车、血压计、洗手盆水龙头、换药桶、听诊器、脉搏血氧仪、呼吸机、除颤器和担架表面采集了235份拭子。按照标准微生物技术对细菌进行分离、鉴定和抗生素敏感性试验。
在235份样本中，有152份样本观察到细菌生长。从235份样本中总共分离出90份样本。本研究纳入的大多数采样部位都被……污染。从床栏表面培养出的……数量最多。在总共……分离株中，54.4%（49/90）为耐甲氧西林……（MRSA）。在8.1%的MRSA分离株（4/49）中检测到万古霉素耐药。……是最常见的革兰氏阴性菌分离株。
记录到重症监护病房的物体/仪器细菌污染率很高。最常见的污染细菌是……，MRSA比例很高且出现了VRSA。定期进行微生物监测、检测污染部位以及有效的去污方法将使潜在病原体的定植及其传播降至最低。
