Pak J Biol Sci. 2022 Jan;25(4):353-357. doi: 10.3923/pjbs.2022.353.357.
<b>Background and Objective:</b> The housefly, <i>Musca domestica</i> L. is the most widespread species of fly in the world. It is in close association with many human pathogens that which can cause serious and life-threatening diseases are known to be carried by house flies, including bacteria, viruses, fungi and parasites, Therefore, this study came intending to determine the role of house flies isolated from some slaughterhouses in the city of Al-Diwaniyah/Iraq in the spread of bacterial and fungal pathogens to humans. <b>Materials and Methods:</b> The 120 insects of house flies were collected randomly from some slaughterhouses for the period September to November, 2021 by special networks prepared for this purpose and transferred to the laboratory and then the bacteria and fungi were isolated and identified from the external surface of house flies by using appropriate culture media. <b>Results:</b> In this study, 148 bacterial isolates belonging to 15 species of bacteria were isolated and diagnosed from the external body of house flies that isolated, 91 samples had given positive growth of bacteria and <i>Escherichia coli</i> was the most occurrence and frequent from the other bacterial isolates, the occurrence rate of it was 16/91 (17.582%) with a frequency rate of 27/148 (18.243%). The 154 fungal isolates belonging to 16 species of fungi were isolated and diagnosed from the external body of house flies, 87 samples had given a positive growth of fungi and <i>Aspergillus niger</i> was the most occurrence and frequent from the other fungal isolates, as its occurrence rate reached 14/87 (16.091%) and the frequency rate of 21/154 (13.636%). <b>Conclusion:</b> House flies transmit many pathological microorganisms such as bacteria, fungi, viruses, etc., which causes various diseases for humans and animals and it may help the spread of antibiotic-resistant species and there is an urgent need to conduct many studies to know the new types of microorganisms that house flies can transmit.
<b>背景和目的：</b>家蝇，<i>Musca domestica</i>L. 是世界上分布最广泛的蝇种。它与许多人类病原体密切相关，已知这些病原体可通过家蝇传播，包括细菌、病毒、真菌和寄生虫，因此，本研究旨在确定从伊拉克迪瓦尼耶市的一些屠宰场分离的家蝇在传播细菌和真菌病原体方面对人类的作用。<b>材料和方法：</b>2021 年 9 月至 11 月，通过为此目的准备的特殊网络，从一些屠宰场随机收集了 120 只家蝇昆虫，将其转移到实验室，然后使用适当的培养基从家蝇的外表面分离和鉴定细菌和真菌。<b>结果：</b>在这项研究中，从分离出的家蝇体外共分离和诊断出 148 株属于 15 种细菌的细菌，91 个样本中细菌生长呈阳性，<i>大肠杆菌</i>是最常见和最频繁的细菌分离株，其检出率为 16/91（17.582%），频率率为 27/148（18.243%）。从家蝇体外分离和诊断出 154 株属于 16 种真菌的真菌，87 个样本中真菌生长呈阳性，<i>黑曲霉</i>是最常见和最频繁的真菌分离株，其检出率为 14/87（16.091%），频率率为 21/154（13.636%）。<b>结论：</b>家蝇传播许多病理微生物，如细菌、真菌、病毒等，这些微生物会导致人类和动物的各种疾病，它可能有助于传播抗生素耐药物种，因此迫切需要开展更多的研究来了解家蝇可以传播的新型微生物。
