Laboratorio de Investigación en Biología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú.
Laboratorio de Biotecnología, Facultad de Ciencias Naturales y Matemática, Universidad Nacional Federico Villarreal, Lima, Perú.
Rev Peru Med Exp Salud Publica. 2024 Aug 19;41(2):164-170. doi: 10.17843/rpmesp.2024.412.13257.
Motivation for the study. The presence of antibiotic resistance genes in bacteria isolated from common flies is a potential public health hazard because it facilitates the presence and spread of antibiotic resistance genes in the environment. Main findings. Thirty-eight bacterial strains identified in 14 species were isolated from within the fly bodies, of which 31 strains showed resistance to carbapenems and 26 strains showed resistance to colistin. Seven bacterial strains showed carbapenem resistance genes and one Escherichia coli strain had resistance to KPC, OXA-48 and mcr-1. Implications. This is the first report of antibiotic resistance genes in bacteria carried by common flies in Peru. The objective was to determine the presence of carbapenem resistance genes and plasmid resistance to colistin (mcr-1) in bacteria isolated from Musca domestica in a garbage dump near a hospital in Lima, Peru. Bacteria with phenotypic resistance to carbapenemics were isolated on CHROMagar mSuperCARBATM medium and colistin resistance profiling was performed using the colistin disk elution method. Detection of blaKPC, blaNDM, blaIMP, blaOXA-48, blaVIM and mcr-1 genes was performed by conventional PCR. The antimicrobial susceptibility profile was determined using the automated MicroScan system. We found that 31/38 strains had phenotypic resistance to carbapenemics and 26/38 strains had phenotypic resistance to colistin with a minimum inhibitory concentration ≥ 4 µg/ml. Finally, we identified seven bacterial strains with carbapenem resistance genes (OXA-48 and KPC) and one bacterial strain with plasmid resistance to colistin (mcr-1). One Escherichia coli strain had three resistance genes: KPC, OXA-48 and mcr-1.
研究动机。从常见苍蝇中分离出的细菌中存在抗生素耐药基因，这是一个潜在的公共卫生危害，因为它促进了环境中抗生素耐药基因的存在和传播。主要发现。从苍蝇体内分离出的 14 个种的 38 株细菌菌株，其中 31 株对碳青霉烯类药物表现出耐药性，26 株对黏菌素表现出耐药性。7 株细菌菌株表现出碳青霉烯类耐药基因，1 株大肠杆菌菌株对 KPC、OXA-48 和 mcr-1 具有耐药性。结论。这是首次在秘鲁报道常见苍蝇携带的细菌中存在抗生素耐药基因。本研究旨在确定从秘鲁利马一家医院附近垃圾场的家蝇中分离的细菌中是否存在碳青霉烯类耐药基因和质粒对黏菌素（mcr-1）的耐药性。在 CHROMagar mSuperCARBATM 培养基上分离出对碳青霉烯类药物表型耐药的细菌，并采用黏菌素药敏纸片洗脱法进行黏菌素耐药表型分析。采用常规 PCR 检测 blaKPC、blaNDM、blaIMP、blaOXA-48、blaVIM 和 mcr-1 基因。采用自动化 MicroScan 系统测定抗菌药物敏感性谱。我们发现，31/38 株细菌对碳青霉烯类药物表现出表型耐药，26/38 株细菌对最低抑菌浓度≥4µg/ml 的黏菌素表现出表型耐药。最后，我们鉴定出 7 株具有碳青霉烯类耐药基因（OXA-48 和 KPC）的细菌菌株和 1 株具有质粒对黏菌素耐药（mcr-1）的细菌菌株。1 株大肠杆菌菌株有 3 种耐药基因：KPC、OXA-48 和 mcr-1。
