冷冻保存后的造血祖细胞移植物的活力不能预测异基因造血干细胞移植中的延迟植入。
The Viability of Hematopoietic Progenitor Cell Grafts after Cryopreservation Does Not Predict Delayed Engraftment in Allogeneic Hematopoietic Stem Cell Transplantation.
机构信息
Wake Forest University School of Medicine - Pathology, Winston-Salem, North Carolina, USA.
Wake Forest University School of Medicine - Anesthesiology, Winston-Salem, North Carolina, USA.
出版信息
Lab Med. 2022 Sep 1;53(5):509-513. doi: 10.1093/labmed/lmac042.
OBJECTIVE
Due to the COVID-19 pandemic, more peripheral blood stem cell (PBSC) allogeneic grafts are being frozen and infused thawed. Our objective was to study the influence of graft viability on engraftment outcome in patients treated with PBSCs.
METHODS
Using trypan blue stain, we compared total nucleated cell (TNC) viability of both fresh and thawed grafts in allogeneic PBSCs.
RESULTS
The viability of thawed PBSC grafts median was 74%, and fresh was 99.0%. The median number of CD34 + cells/kg infused thawed was 6.3 × 106/kg and median time to neutrophil and platelet engraftment was 17.5 and 20 days. Median number of CD34 + cells/kg infused fresh was 5.1 × 106/kg and median time to neutrophil and platelet engraftment was 18 and 19 days. There were no statistically significant differences in the time to engraftment between the 2 groups.
CONCLUSION
A low TNC viability of thawed PBSC grafts does not have an effect on time to neutrophil and platelet engraftment when more than 2.85 × 106 CD34 + cells/kg are infused.
目的
由于 COVID-19 大流行，更多的外周血干细胞（PBSC）异基因移植物被冷冻并输注解冻。我们的目的是研究在接受 PBSC 治疗的患者中，移植物活力对植入结果的影响。
方法
使用台盼蓝染色，我们比较了新鲜和解冻的异基因 PBSC 中的总核细胞（TNC）活力。
结果
解冻 PBSC 移植物的活力中位数为 74%，新鲜的为 99.0%。解冻时输注的 CD34+细胞/kg 的中位数为 6.3×106/kg，中性粒细胞和血小板植入的中位数时间为 17.5 和 20 天。新鲜时输注的 CD34+细胞/kg 的中位数为 5.1×106/kg，中性粒细胞和血小板植入的中位数时间为 18 和 19 天。两组间植入时间无统计学差异。
结论
当输注超过 2.85×106 CD34+细胞/kg 时，解冻 PBSC 移植物的低 TNC 活力对中性粒细胞和血小板植入时间没有影响。
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