Durand-Clement M
Toxicon. 1986;24(11-12):1153-7. doi: 10.1016/0041-0101(86)90141-8.
The dinoflagellate Gambierdiscus toxicus was cultivated under different environmental conditions. By an improved toxin extraction procedure the toxicity of methanol extracts was evaluated. Medium composition, temperature decrease, origin (clonal or not clonal) and culture age did not significantly modify toxin production. The discharge of trichocysts and the abundance of bacteria did not affect the toxicity of the dinoflagellate. The toxin yield of G. toxicus cultures was quite stable and a mean of 1100 cells were sufficient to kill one 20 g mouse within 24 hr.
在不同环境条件下培养了有毒的甲藻甘比甲藻。通过改进的毒素提取程序，评估了甲醇提取物的毒性。培养基成分、温度降低、来源（克隆或非克隆）和培养年龄对毒素产生没有显著影响。刺丝泡的释放和细菌的丰度不影响甲藻的毒性。甘比甲藻培养物的毒素产量相当稳定，平均1100个细胞足以在24小时内杀死一只20克重的小鼠。
