Holmes M J, Lewis R J, Gillespie N C
Southern Fisheries Research Centre, Queensland Department of Primary Industries, Australia.
Toxicon. 1990;28(10):1159-72. doi: 10.1016/0041-0101(90)90116-o.
Gambierdiscus toxicus strains isolated from Australia and French Polynesia were grown in modified f2 and f10 nutrient media and the cells extracted for ciguatoxin and maitotoxin. The high nutrient enrichment of f2 media induced aberrant cell morphology in both strains whereas the majority of cells grown in f10 media had the typical lenticular shape of wild G. toxicus cells. The Australian strain grew faster and produced greater cell densities than the French Polynesian strain. Different chromatographic types of maitotoxin were extracted from the two G. toxicus strains and purified to homogeneity using reverse-phase high-performance liquid chromatography. The toxins elicited similar bioassay signs in mice, but produced different death-time vs dose relationships. The maitotoxin extracted from the Australian strain eluted later on both straight-phase and reverse-phase chromatographic columns than did the maitotoxin extracted from the French Polynesian strain. The maitotoxin extracted from the French Polynesian strain was chromatographically identical to reference maitotoxin. For each strain no differences were found between maitotoxins extracted from cells grown in f2 or f10 media. Only one toxin was produced by each strain of G. toxicus. Ciguatoxin was not produced by either strain.
从澳大利亚和法属波利尼西亚分离出的甘比毒藻菌株在改良的f2和f10营养培养基中培养，然后提取细胞中的雪卡毒素和刺尾鱼毒素。f2培养基的高营养富集导致两种菌株的细胞形态异常，而在f10培养基中生长的大多数细胞具有野生甘比毒藻细胞典型的透镜状形状。澳大利亚菌株比法属波利尼西亚菌株生长得更快，细胞密度更高。从两种甘比毒藻菌株中提取了不同色谱类型的刺尾鱼毒素，并使用反相高效液相色谱法纯化至同质。这些毒素在小鼠身上引发了相似的生物测定体征，但产生了不同的死亡时间与剂量关系。从澳大利亚菌株中提取的刺尾鱼毒素在正相和反相色谱柱上的洗脱时间都比从法属波利尼西亚菌株中提取的刺尾鱼毒素晚。从法属波利尼西亚菌株中提取的刺尾鱼毒素在色谱上与参考刺尾鱼毒素相同。对于每种菌株，从在f2或f10培养基中生长的细胞中提取的刺尾鱼毒素之间没有发现差异。每种甘比毒藻菌株只产生一种毒素。两种菌株均未产生雪卡毒素。
