Institute of Agri-Food, Animal and Environmental Sciences (ICA3), Universidad de O'Higgins, Campus Colchagua, Ruta 90, KM 3, San Fernando, Chile.
Advanced Center for Chronic Diseases (ACCDiS), Santiago, Chile.
Mycotoxin Res. 2022 Aug;38(3):185-191. doi: 10.1007/s12550-022-00459-x. Epub 2022 May 31.
We assessed the occurrence of aflatoxin M (AFM) in urine in a sample of the MAUCO population-based cohort (n = 120) using an enzyme-linked immune sorbent assay (ELISA) kit specially designed for the analysis of AFM in urine. We found AFM in the urine of 59% of the participants (> limit of detection), with 12% of the samples being over the limit of quantification. The mean of the quantifiable samples was 0.66 (± 0.35) ng/mg adjusted creatinine, ranging from 0.31 to 1.39 ng/mg creatinine. The mean probable daily intake (PDI) of AFB was 0.23 (± 0.37) ng/kg bw according to the upper bound (UB), being significantly higher in women and 0.14 (± 0.23) ng/kg bw in the modified lower bound (mLB) approach, ranging from 0.01 to 1.98 ng/kg bw. The risk of AFB was assessed with the margin of exposure (MOE) approach estimated at 2800 in the mean mLB and 1733 in the mean UB. According to the MOE values obtained in this study, aflatoxin B exposure must be considered a public health concern and must be taken as a priority for food risk management.
我们使用专门设计用于尿液中 AFM 分析的酶联免疫吸附测定（ELISA）试剂盒，评估了 MAUCO 基于人群的队列（n=120）中尿液中黄曲霉毒素 M（AFM）的发生情况。我们发现 59%的参与者（>检测限）尿液中存在 AFM，其中 12%的样本超过了定量限。可定量样本的平均值为 0.66（±0.35）ng/mg 校正肌酐，范围为 0.31 至 1.39 ng/mg 肌酐。根据上限（UB），AFB 的平均可能每日摄入量（PDI）为 0.23（±0.37）ng/kg bw，女性显著更高，改良下限（mLB）方法为 0.14（±0.23）ng/kg bw，范围为 0.01 至 1.98 ng/kg bw。我们使用暴露量与安全系数（MOE）评估方法，mLB 平均值估计为 2800，UB 平均值为 1733。根据本研究中获得的 MOE 值，黄曲霉毒素 B 暴露必须被视为公共卫生问题，必须优先考虑进行食品风险管理。
