Matsuno T
Vet Parasitol. 1986 Dec;22(3-4):249-58. doi: 10.1016/0304-4017(86)90112-3.
In a series of battery experiments utilizing 9-day-old White Leghorn male chicks, 9-(2-chloro-6-fluorobenzyl)-6-methylaminopurine (VM 6387) and 9-(2-chloro-6-fluorobenzyl)-6-dimethylaminopurine (VM 6736) showed remarkable anticoccidial activity against Eimeria tenella, E. acervulina, and E. maxima. The effective dose range was estimated from the results of the efficacy test against E. tenella on the basis of improvement of body weight gain and clinical signs of infection. The tests included dietary levels of 60-100 ppm of VM 6387 and 70-110 ppm of VM 6736. Both compounds gave good protection against E. acervulina and E. maxima at the same dose range. The sporulation of oocysts obtained from cecal contents of birds fed lower levels of VM 6387 was inhibited. Studies of the effects of VM 6387 on stages of the E. tenella life cycle demonstrated that the compound possesses remarkable activity at 1-5 days post-infection corresponding to the period of schizogony, as well as prolonged activity thereafter, when the parasite was undergoing gametogenesis.
在一系列使用9日龄白来航雄性雏鸡的组试验中，9-(2-氯-6-氟苄基)-6-甲氨基嘌呤(VM 6387)和9-(2-氯-6-氟苄基)-6-二甲氨基嘌呤(VM 6736)对柔嫩艾美耳球虫、堆型艾美耳球虫和巨型艾美耳球虫显示出显著的抗球虫活性。根据对柔嫩艾美耳球虫药效试验结果，基于体重增加和感染临床症状的改善情况，估算有效剂量范围。试验包括60 - 100 ppm的VM 6387和70 - 110 ppm的VM 6736的日粮水平。两种化合物在相同剂量范围内对堆型艾美耳球虫和巨型艾美耳球虫都有良好的保护作用。从饲喂较低水平VM 6387的鸡盲肠内容物中获得的卵囊孢子化受到抑制。对VM 6387对柔嫩艾美耳球虫生命周期各阶段影响的研究表明，该化合物在感染后1 - 5天对应于裂殖生殖期具有显著活性，此后在寄生虫进行配子生殖时也具有延长的活性。
