Matsuno T, Kobayashi N, Hariguchi F, Yamazaki T, Imai K, Onaga H
Exp Parasitol. 1984 Feb;57(1):55-61. doi: 10.1016/0014-4894(84)90062-6.
The anticoccidial activity of an orotic acid analog, 1,6-dihydro-6-oxo-2-pyrazinecarboxylic acid 4-oxide (carboxyemimycin), was tested in battery experiments, utilizing 9-day-old Single-Comb White Leghorn cockerels. Carboxyemimycin, at 125 ppm and more in feed, exhibited marked anticoccidial activities against Eimeria tenella, E. necatrix, E. acervulina, and E. maxima. High doses of carboxyemimycin--up to 1000 ppm--did not cause any reduction in weight gains. The battery and in vitro studies with delayed and restricted medications revealed that carboxyemimycin affected the development of E. tenella in first and second generation schizogony and in gametogony.
利用9日龄单冠白来航公鸡，在一组试验中对乳清酸类似物1,6-二氢-6-氧代-2-吡嗪羧酸4-氧化物（羧基埃米霉素）的抗球虫活性进行了测试。饲料中羧基埃米霉素含量达到125 ppm及以上时，对柔嫩艾美耳球虫、毒害艾美耳球虫、堆型艾美耳球虫和巨型艾美耳球虫表现出显著的抗球虫活性。高剂量的羧基埃米霉素（高达1000 ppm）并未导致体重增加有任何减少。采用延迟给药和限制用药的组试验及体外研究表明，羧基埃米霉素影响柔嫩艾美耳球虫第一代和第二代裂殖生殖以及配子生殖的发育。
