Department of Otorhinolaryngology Head and Neck Surgery, Ningbo Medical Center Lihuili Hospital, Ningbo, Zhejiang, China; Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital affiliated to Ningbo University, Ningbo, Zhejiang, China.
Department of Otorhinolaryngology Head and Neck Surgery, Lihuili Hospital affiliated to Ningbo University, Ningbo, Zhejiang, China.
Genomics. 2022 Jul;114(4):110392. doi: 10.1016/j.ygeno.2022.110392. Epub 2022 May 26.
tRNA-derived small RNAs (tsRNAs) participate in several biological processes, including carcinogenesis. The correlations between tsRNAs and human cancers are attracting substantial attention. Nevertheless, the involvement of tsRNAs in laryngeal squamous cell carcinoma (LSCC) progression remains unclear. We constructed tsRNAs expression profiles in LSCC and adjacent normal tissues by next-generation sequencing. Interestingly, we identified a specific 5'-tiRNA fragment (tRF-33-Q1Q89P9L842205) that was significantly downregulated and was closely associated with lymph node metastasis and advanced stages of LSCC. Importantly, we found that tRF-33-Q1Q89P9L842205 suppressed cell growth, proliferation, migration, invasion and induced apoptosis in LSCC by directly silencing phosphoinositide 3-kinase catalytic subunit (PIK3CD). We speculated that tRF-33-Q1Q89P9L842205 is a potential diagnostic biomarker for LSCC and acts as a tumor suppressor by directly targeting PIK3CD.
tRNA 衍生的小 RNA（tsRNAs）参与多种生物学过程，包括致癌作用。tsRNAs 与人类癌症之间的相关性引起了广泛关注。然而，tsRNAs 在喉鳞状细胞癌（LSCC）进展中的作用尚不清楚。我们通过下一代测序构建了 LSCC 及相邻正常组织中的 tsRNAs 表达谱。有趣的是，我们鉴定出一个特异性的 5'-tiRNA 片段（tRF-33-Q1Q89P9L842205），其表达明显下调，并与 LSCC 的淋巴结转移和晚期密切相关。重要的是，我们发现 tRF-33-Q1Q89P9L842205 通过直接沉默磷酸肌醇 3-激酶催化亚基（PIK3CD），抑制 LSCC 细胞的生长、增殖、迁移、侵袭并诱导凋亡。我们推测 tRF-33-Q1Q89P9L842205 是 LSCC 的潜在诊断生物标志物，并通过直接靶向 PIK3CD 发挥肿瘤抑制作用。
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