Department of Laboratory Medicine, Affiliated Hospital of Nantong University, Nantong, Jiangsu 226006, China.
Medical School of Nantong University, Nantong University, Nantong, Jiangsu 226007, China.
Int J Med Sci. 2023 Aug 6;20(9):1189-1201. doi: 10.7150/ijms.85180. eCollection 2023.
Gastric cancer (GC) has high morbidity and mortality due to inefficient early screening. Therefore, we are searching for more sensitive and specific diagnostic markers for GC. tRNA-derived small RNAs are novel non-coding small RNAs with good abundance and stable presence in body fluids, which may play multiple biological regulatory roles. In this study, we aimed to find a potential biomarker with high accuracy in tRNA-derived small RNAs that can help diagnose GC. tRF-27-FDXXE6XRK45 was screened as a target molecule by high-throughput sequencing in three pairs of GC tissues. RNA quantitative reverse transcription PCR was conducted to detect the expression levels of tRF-27-FDXXE6XRK45. Agarose gel electrophoresis, Sanger sequencing, cytoplasmic and nuclear RNA isolation assays, gradient dilution experiments, and room temperature and repeated freeze-thaw experiments were used to assess the detection performance of tRF-27-FDXXE6XRK45. Using the chi-square test to analyze the correlation between tRF-27-FDXXE6XRK45 expression levels and clinicopathological parameters. In addition, receiver operating characteristic curves were used to evaluate the diagnostic value of tRF-27-FDXXE6XRK45 in GC. tRF-27-FDXXE6XRK45 expression levels, significantly upregulated in tissues and sera of GC patients and decreased after radical GC surgery, were correlated with the degree of differentiation, depth of tumor infiltration, TNM stage, lymph node metastasis, and nerve/vascular invasion. In comparison with current GC diagnostic markers, tRF-27-FDXXE6XRK45 displayed better efficacy. tRF-27-FDXXE6XRK45, with high diagnostic efficacy, can distinguish GC patients from gastritis patients and healthy donors, suggesting that tRF-27-FDXXE6XRK45 may be a promising candidate as a diagnostic marker for GC.
胃癌（GC）由于早期筛查效率低下，发病率和死亡率都很高。因此，我们正在寻找更敏感和特异的 GC 诊断标志物。tRNA 衍生的小 RNA 是一种新型的非编码小 RNA，在体液中具有良好的丰度和稳定性，可能发挥多种生物学调节作用。在本研究中，我们旨在从高通量测序的 3 对 GC 组织中筛选出一种具有高准确性的小 RNA 潜在生物标志物，以帮助诊断 GC。tRF-27-FDXXE6XRK45 通过高通量测序筛选为靶分子。采用 RNA 定量逆转录 PCR 检测 tRF-27-FDXXE6XRK45 的表达水平。琼脂糖凝胶电泳、Sanger 测序、细胞质和核 RNA 分离试验、梯度稀释试验、室温和反复冻融试验评估 tRF-27-FDXXE6XRK45 的检测性能。采用卡方检验分析 tRF-27-FDXXE6XRK45 表达水平与临床病理参数的相关性。此外，采用受试者工作特征曲线评估 tRF-27-FDXXE6XRK45 对 GC 的诊断价值。tRF-27-FDXXE6XRK45 的表达水平在 GC 患者的组织和血清中显著上调，根治性 GC 手术后降低，与分化程度、肿瘤浸润深度、TNM 分期、淋巴结转移、神经/血管侵犯有关。与目前的 GC 诊断标志物相比，tRF-27-FDXXE6XRK45 显示出更好的效果。tRF-27-FDXXE6XRK45 具有较高的诊断效能，可将 GC 患者与胃炎患者和健康供者区分开来，提示 tRF-27-FDXXE6XRK45 可能是 GC 诊断标志物的有前途的候选物。
