Kundu Banani, Reis Rui L, Kundu Subhas C
3B's Research Group, I3Bs-Research Institute on Biomaterials, Biodegradables and Biomimetics, University of Minho, Headquarters of the European Institute of Excellence on Tissue Engineering and Regenerative Medicine, AvePark, Parque de Ciência e Tecnologia, Zona Industrial da Gandra, 4805-017 Barco, Portugal.
ICVS/3B's-PT Government Associate Laboratory, 4710-057 Braga, Portugal.
Biomimetics (Basel). 2022 May 20;7(2):64. doi: 10.3390/biomimetics7020064.
Osteomyelitis is an inflammation of the bone caused by bacterial infection. It usually develops from broken bones, decayed teeth, or heavily punctured wounds. Multi-drug-resistant bacteria are the major hurdle in the treatment of osteomyelitis. The ever-rising antibiotic resistance even leads to amputations or fatalities as a consequence of chronic osteomyelitis. Hence, a single agent with antibacterial activity as well as bone regenerative properties can serve as a potential off-the-shelf product in the treatment of osteomyelitis. Herein, the antibacterial and pro-osteogenic characteristics of copper sericin (Cu-SER) metal-organic frameworks (MOFs) are reported. Sericin, a silk protein with antibacterial activity and an osteoinduction property, acts as an organic template for the deposition of Cu-SER MOFs, similar to collagen during biomineralization in bone. The MOFs exhibit cytocompatibility and osteogenic activity in a dose-dependent manner, as revealed by cell proliferation (alamarBlue) and mineralization (Alizarin Red S and Energy Dispersive X-ray analysis). The bactericidal activity of Cu-SER MOFs was investigated by scanning electron microscopy and a growth kinetic analysis. Together, the report illuminates the unique phenomenon of Cu-SER MOFs that kill bacteria upon contact while being well-tolerated by primary human cells. Hence, Cu-SER MOFs hold the potential to minimize antibiotic dependence.
骨髓炎是由细菌感染引起的骨炎症。它通常由骨折、龋齿或严重刺伤发展而来。耐多药细菌是治疗骨髓炎的主要障碍。不断上升的抗生素耐药性甚至会导致因慢性骨髓炎而截肢或死亡。因此，一种具有抗菌活性以及骨再生特性的单一药物可以作为治疗骨髓炎的潜在现货产品。在此，报道了铜丝胶蛋白（Cu-SER）金属有机框架（MOF）的抗菌和成骨特性。丝胶蛋白是一种具有抗菌活性和骨诱导特性的丝蛋白，它作为Cu-SER MOF沉积的有机模板，类似于骨生物矿化过程中的胶原蛋白。如细胞增殖（alamarBlue）和矿化（茜素红S和能量色散X射线分析）所示，MOF以剂量依赖的方式表现出细胞相容性和成骨活性。通过扫描电子显微镜和生长动力学分析研究了Cu-SER MOF的杀菌活性。总之，该报告揭示了Cu-SER MOF的独特现象，即接触后能杀死细菌，同时能被原代人类细胞良好耐受。因此，Cu-SER MOF有潜力将抗生素依赖性降至最低。
