Kaliyadan Feroze, Jayasree Puravoor, Ashique Karalikkattil T
King Faisal University, Saudi Arabia.
Medical Trust Hospital, India.
Postepy Dermatol Alergol. 2022 Apr;39(2):304-306. doi: 10.5114/ada.2021.102850. Epub 2021 Jan 20.
Repeated open application testing (ROAT) is an important adjuvant in the evaluation of allergic contact dermatitis. There are variations in the way ROAT is used by practitioners and standardizing the method, in terms of the area and amount of the antigen applied, could help improve the validity of the process.
Our study attempts to address ways in which part of the ROAT technique can be standardized - with respect to the area and amount of application and validation of the same through consensus opinion.
Two proposed modifications - one for the area of application (using waterproof surgical markers and a stencil to mark the area of application) and one for daily amount applied (using easily available 1 cm syringes), for the ROAT technique were drafted. The same was discussed with a total of 10 dermatologists. The participants were given four statements and were asked to choose one option for each statement (strongly agree - agree - neutral - disagree - strongly disagree). All the respondents were also asked to give their opinion on possible limitations and problems of the proposed modifications and possible solutions for the same.
There was generally a consensus among the 10 dermatologists, with the majority agreeing that the proposed modifications were both effective and practical.
Simple practical modifications of the ROAT technique like marking the area to be applied using a waterproof skin marker and stencil, and the use of 1 cm syringes to dispense the test product, can improve the standardization of the process.
重复开放涂抹试验（ROAT）是评估过敏性接触性皮炎的一项重要辅助手段。临床医生使用ROAT的方式存在差异，在涂抹抗原的面积和用量方面规范该方法，有助于提高检测过程的有效性。
我们的研究试图探讨使ROAT技术的部分环节标准化的方法——在涂抹面积和用量方面，并通过达成共识来验证其有效性。
针对ROAT技术起草了两项改进建议——一项是关于涂抹面积（使用防水手术标记笔和模板标记涂抹区域），另一项是关于每日用量（使用易于获取的1厘米注射器）。与总共10名皮肤科医生讨论了这些建议。参与者收到四条陈述，并被要求为每条陈述选择一个选项（强烈同意——同意——中立——不同意——强烈不同意）。所有受访者还被要求就所提议改进措施可能存在的局限性和问题以及相应的可能解决方案发表意见。
10名皮肤科医生总体上达成了共识，大多数人认为所提议的改进措施既有效又实用。
对ROAT技术进行简单实用的改进，如使用防水皮肤标记笔和模板标记涂抹区域，以及使用1厘米注射器 dispense 测试产品，可以提高检测过程的标准化程度。 （原文中“dispense”这里翻译为“分配、分发”，结合语境就是用注射器分发测试产品，由于不确定具体含义，可根据实际情况调整。）
