Tehseen Muhammad Massub, Tonk Fatma Aykut, Tosun Muzaffer, Randhawa Harpinder Singh, Kurtulus Ezgi, Ozseven Izzet, Akin Behyan, Nur Zulfuagaoglu Ozge, Nazari Kumarse
Department of Field Crops, Ege University, Izmir, Turkey.
Agriculture and Agri-Food Canada, Lethbridge, AB, Canada.
Front Genet. 2022 May 11;13:900558. doi: 10.3389/fgene.2022.900558. eCollection 2022.
Stripe rust caused by f. sp. . is a major bread wheat disease worldwide with yield losses of up to 100% under severe disease pressure. The deployment of resistant cultivars with adult plant resistance to the disease provides a long-term solution to stripe rust of wheat. An advanced line from the International Winter Wheat Improvement Program (IWWIP) 130675 (Avd/Vee#1//1-27-6275/Cf 1770/3/MV171-C-17466) showed a high level of adult plant resistance to stripe rust in the field. To identify the adult plant resistance genes in this elite line, a mapping population of 190 doubled haploid (DH) lines was developed from a cross between line 130675 and the universal stripe rust-susceptible variety Avocet S. The DH population was evaluated at precision wheat stripe rust phenotyping platform, in Izmir during 2019, 2020, and 2021 cropping seasons under artificial inoculations. Composite interval mapping (CIM) identified two stable , and , which were detected in multiple years. In addition to these two , five more , , , , , and , were identified, which were specific to the cropping year (environment). All were derived from the resistant parent, except . The significant QTLs explained 3.4-20.6% of the phenotypic variance. SNP markers flanking the regions can be amenable to marker-assisted selection. The best DH lines with high yield, end-use quality, and stripe rust resistance can be used for further selection for improved germplasm. SNP markers flanking the QTL regions can aid in identifying such lines.
由小麦条锈菌引起的条锈病是全球范围内面包小麦的主要病害，在严重病害压力下产量损失可达100%。部署对该病具有成株抗性的抗病品种为小麦条锈病提供了长期解决方案。国际冬小麦改良计划（IWWIP）的一个先进品系130675（Avd/Vee#1//1 - 27 - 6275/Cf 1770/3/MV171 - C - 17466）在田间表现出对条锈病的高水平成株抗性。为了鉴定该优良品系中的成株抗性基因，从品系130675与通用的条锈病感病品种阿沃塞特S杂交后代中构建了一个包含190个双单倍体（DH）系的作图群体。在2019年、2020年和2021年种植季节，在伊兹密尔的精准小麦条锈病表型鉴定平台上对该DH群体进行人工接种评估。复合区间作图（CIM）鉴定出两个稳定的QTL，即QYr.iari - 2B.1和QYr.iari - 2B.2，它们在多个年份被检测到。除了这两个QTL外，还鉴定出另外五个QTL，即QYr.iari - 2B.3、QYr.iari - 2B.4、QYr.iari - 2B.5、QYr.iari - 2B.6和QYr.iari - 2B.7，它们是特定于种植年份（环境）的。所有QTL均来自抗性亲本，除了QYr.iari - 2B.7。这些显著的QTL解释了3.4% - 20.6%的表型变异。QTL区域两侧的SNP标记可用于标记辅助选择。具有高产、最终用途品质和条锈病抗性的最佳DH系可用于进一步选择改良种质。QTL区域两侧的SNP标记有助于鉴定此类品系。
