Laboratory Medicine Center, Department of Clinical Laboratory, Zhejiang Provincial People's Hospital, Affiliated People's Hospital, Hangzhou Medical College, Hangzhou, China.
Department of Respiratory Medicine, Yuhang Second People's Hospital, Hangzhou, China.
Front Cell Infect Microbiol. 2022 May 12;12:902774. doi: 10.3389/fcimb.2022.902774. eCollection 2022.
To characterize two plasmids co-harboring carbapenem resistance genes and in carbapenem-resistant (CRKP) strains.
Two clinical CRKP strains were isolated and characterized by antimicrobial susceptibility testing, conjugation assays, whole-genome sequencing, and bioinformatics analysis.
The two CRKP strains NB4 and NB5 were both resistant to imipenem, meropenem and tigecycline. Whole-genome sequencing revealed that two CRKP strains belonged to the ST11 type and carried multiple resistance genes. The clusters in both strains were located on the IncFIB(Mar)-like/HI1B-like group of hybrid plasmids, which co-harbored the metallo-β-lactamase gene . In addition, the co-existence of and and the presence of in CRKP strain NB5 was observed.
In this study, gene clusters were identified in two NDM-1-producing CRKP ST11 strains. These gene clusters will likely spread into clinical high-risk CRKP clones and exacerbate the antimicrobial resistance crisis. In addition, we detected the co-occurrence of , and in a single strain, which will undoubtedly accelerate the formation of a "superdrug resistant" bacteria. Hence, effective control measures should be implemented to prevent the further dissemination of such organisms in clinical settings.
研究两种同时携带碳青霉烯类耐药基因和 的质粒在碳青霉烯类耐药 （CRKP）菌株中的特征。
从临床分离出两株 CRKP 菌株，并通过药敏试验、接合试验、全基因组测序和生物信息学分析对其进行了表征。
两株 CRKP 菌株 NB4 和 NB5 均对亚胺培南、美罗培南和替加环素耐药。全基因组测序显示，两株 CRKP 菌株均属于 ST11 型，携带多种耐药基因。两个 簇均位于 IncFIB(Mar)-样/HI1B-样杂合质粒群上，共同携带金属β-内酰胺酶基因 。此外，还观察到 CRKP 菌株 NB5 中 和 的共存以及 的存在。
本研究在两株产 NDM-1 的 CRKP ST11 菌株中鉴定出了 基因簇。这些基因簇可能会传播到临床高危 CRKP 克隆中，加剧抗菌药物耐药危机。此外，我们在单个菌株中检测到 、 和 的同时存在，这无疑将加速“超级耐药菌”的形成。因此，应采取有效的控制措施，防止此类生物体在临床环境中进一步传播。
