Coexistence of plasmid-mediated , , and in .

is a potential pathogen that has not been studied comprehensively. The emergence of multidrug-resistant (MDR) , specifically strains resistant to tigecycline and carbapenem, presents a significant challenge to clinical treatment. This investigation aimed to characterize MDR strain FK8966, co-carrying , , and by plasmids. It was observed that FK8966's MDR was primarily because of the IncHI1B-like plasmid co-carrying and , and an IncFIB(K)/IncFII(K) plasmid harboring . Furthermore, the phylogenetic analysis revealed that IncHI1B-like plasmids carrying were disseminated among different bacteria, specifically in China. Additionally, according to the comparative genomic analysis, the MDR regions indicated that the gene cluster was inserted into the gene, while was present in transposon Tn linked to the class 1 integron (). It was also observed that an ΔTn insertion with made a novel harboring IncFIB(K)/IncFII(K) plasmid. The antimicrobial resistance prevalence and phylogenetic analyses of strains indicated that FK8966 is a distinct MDR branch of . Furthermore, CRISPR-Cas system analysis showed that many CRISPR-Cas systems lacked spacers matching the two aforementioned novel resistance plasmids, suggesting that these resistance plasmids have the potential to disseminate within . Therefore, the spread of MDR and plasmids warrants further attention.IMPORTANCEThe emergence of multidrug-resistant poses a great threat to clinical care, and the situation is exacerbated by the dissemination of tigecycline- and carbapenem-resistant genes. Therefore, monitoring these pathogens and their resistance plasmids is urgent and crucial. This study identified tigecycline- and carbapenem-resistant strain, FK8966. Furthermore, it is the first study to report the coexistence of , , and in . Moreover, the CRISPR-Cas system of many lacks spacers that match the plasmids carried by FK8966, which are crucial for mediating resistance against tigecycline and carbapenems, indicating their potential to disseminate within .