Liu Xing, Song Ya-Jun, Chen Xing, Huang Meng-Ya, Zhao Chen-Xi, Zhou Xun, Zhou Xin
Department of Cosmetology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China.
Department of Urology, Xinqiao Hospital, The Army Medical University, Chongqing, China.
Front Bioeng Biotechnol. 2022 May 12;10:810244. doi: 10.3389/fbioe.2022.810244. eCollection 2022.
Capsular contracture caused by silicone rubber is a critical issue in plastic surgery that urgently needs to be solved. Studies have shown that carbon ion implant in silicone rubber (carbon silicone rubber, C-SR) can significantly improve the capsular structure, but the effect of this improvement only appear 2months or later. In this study, asiaticoside combined with carbon silicone rubber was used to explore the changes in the capsule to provide a reference for the treatment of capsule contracture. Human fibroblasts (HFF-1) were used for experiments. The combined effect of asiaticoside and carbon silicone rubber on cell proliferation was determined by the CCK8 method, cell migration changes were measured by Transwell assays, cell cycle changes were measured by flow cytometry, and the expression levels of fibroblast transformation markers (vimentin and α-SMA), collagen (Col-1A1) and TGF-β/Smad signaling pathway-related proteins (TGF-β1, TβRI, TβRII and Smad2/3) were detected by immunofluorescence. experiments were carried out by subcutaneous implantation of the material in SD rats, and asiaticoside was oral administered simultaneously. WB and ELISA were used to detect changes in the expression of TGF-β/Smad signaling pathway-related proteins. TGF-β/Smad signaling pathway proteins were then detected and confirmed by HE, Masson and immunohistochemical staining. The results shown that asiaticoside combined with carbon ion implantation inhibited the viability, proliferation and migration of fibroblasts on silicone rubber. immunofluorescence showed that the secretion levels of α-SMA and Col-1A1 were significantly decreased, the transformation of fibroblasts into myofibroblasts was weakened, and the TGF-β/Smad signaling pathway was inhibited. experimental results showed that asiaticoside combined with carbon silicone rubber inhibited TGF-β1 secretion and inhibited the TGF-β/Smad signaling pathway, reducing the thickness of the capsule and collagen deposition. These results imply that carbon silicone rubber combined with asiaticoside can regulate the viability, proliferation and migration of fibroblasts by inhibiting the TGF-β/Smad signaling pathway and reduce capsule thickness and collagen deposition, which greatly reduces the incidence of capsule contracture.
硅胶引起的包膜挛缩是整形外科中亟待解决的关键问题。研究表明，在硅胶中植入碳离子（碳硅胶，C-SR）可显著改善包膜结构，但这种改善效果在2个月或更久之后才会显现。在本研究中，采用积雪草苷联合碳硅胶来探究包膜的变化，为包膜挛缩的治疗提供参考。使用人成纤维细胞（HFF-1）进行实验。采用CCK8法测定积雪草苷和碳硅胶对细胞增殖的联合作用，通过Transwell实验检测细胞迁移变化，用流式细胞术检测细胞周期变化，通过免疫荧光检测成纤维细胞转化标志物（波形蛋白和α-SMA）、胶原蛋白（Col-1A1）以及TGF-β/Smad信号通路相关蛋白（TGF-β1、TβRI、TβRII和Smad2/3）的表达水平。通过将材料皮下植入SD大鼠体内进行实验，并同时口服积雪草苷。采用WB和ELISA检测TGF-β/Smad信号通路相关蛋白表达的变化。然后通过HE、Masson和免疫组织化学染色检测并确认TGF-β/Smad信号通路蛋白。结果显示，积雪草苷联合碳离子植入可抑制硅胶上成纤维细胞的活力、增殖和迁移。免疫荧光显示，α-SMA和Col-1A1的分泌水平显著降低，成纤维细胞向肌成纤维细胞的转化减弱，且TGF-β/Smad信号通路受到抑制。实验结果表明，积雪草苷联合碳硅胶可抑制TGF-β1分泌并抑制TGF-β/Smad信号通路，减少包膜厚度和胶原蛋白沉积。这些结果表明，碳硅胶联合积雪草苷可通过抑制TGF-β/Smad信号通路来调节成纤维细胞的活力、增殖和迁移，并减少包膜厚度和胶原蛋白沉积，从而大大降低包膜挛缩的发生率。
