文献检索文档翻译深度研究
Suppr Zotero 插件Zotero 插件
邀请有礼套餐&价格历史记录

新学期，新优惠

限时优惠:9月1日-9月22日

30天高级会员仅需29元

1天体验卡首发特惠仅需5.99元

了解详情
不再提醒
插件&应用
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
高级版
套餐订阅购买积分包
AI 工具
文献检索文档翻译深度研究
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2025

积雪草苷联合碳离子注入改善硅橡胶生物相容性并降低包膜挛缩风险

Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture.

作者信息

Liu Xing, Song Ya-Jun, Chen Xing, Huang Meng-Ya, Zhao Chen-Xi, Zhou Xun, Zhou Xin

机构信息

Department of Cosmetology, Chongqing Hospital of Traditional Chinese Medicine, Chongqing, China.

Department of Urology, Xinqiao Hospital, The Army Medical University, Chongqing, China.

出版信息

Front Bioeng Biotechnol. 2022 May 12;10:810244. doi: 10.3389/fbioe.2022.810244. eCollection 2022.

DOI:10.3389/fbioe.2022.810244
PMID:35646845
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9133697/
Abstract

Capsular contracture caused by silicone rubber is a critical issue in plastic surgery that urgently needs to be solved. Studies have shown that carbon ion implant in silicone rubber (carbon silicone rubber, C-SR) can significantly improve the capsular structure, but the effect of this improvement only appear 2months or later. In this study, asiaticoside combined with carbon silicone rubber was used to explore the changes in the capsule to provide a reference for the treatment of capsule contracture. Human fibroblasts (HFF-1) were used for experiments. The combined effect of asiaticoside and carbon silicone rubber on cell proliferation was determined by the CCK8 method, cell migration changes were measured by Transwell assays, cell cycle changes were measured by flow cytometry, and the expression levels of fibroblast transformation markers (vimentin and α-SMA), collagen (Col-1A1) and TGF-β/Smad signaling pathway-related proteins (TGF-β1, TβRI, TβRII and Smad2/3) were detected by immunofluorescence. experiments were carried out by subcutaneous implantation of the material in SD rats, and asiaticoside was oral administered simultaneously. WB and ELISA were used to detect changes in the expression of TGF-β/Smad signaling pathway-related proteins. TGF-β/Smad signaling pathway proteins were then detected and confirmed by HE, Masson and immunohistochemical staining. The results shown that asiaticoside combined with carbon ion implantation inhibited the viability, proliferation and migration of fibroblasts on silicone rubber. immunofluorescence showed that the secretion levels of α-SMA and Col-1A1 were significantly decreased, the transformation of fibroblasts into myofibroblasts was weakened, and the TGF-β/Smad signaling pathway was inhibited. experimental results showed that asiaticoside combined with carbon silicone rubber inhibited TGF-β1 secretion and inhibited the TGF-β/Smad signaling pathway, reducing the thickness of the capsule and collagen deposition. These results imply that carbon silicone rubber combined with asiaticoside can regulate the viability, proliferation and migration of fibroblasts by inhibiting the TGF-β/Smad signaling pathway and reduce capsule thickness and collagen deposition, which greatly reduces the incidence of capsule contracture.

摘要

硅胶引起的包膜挛缩是整形外科中亟待解决的关键问题。研究表明，在硅胶中植入碳离子（碳硅胶，C-SR）可显著改善包膜结构，但这种改善效果在2个月或更久之后才会显现。在本研究中，采用积雪草苷联合碳硅胶来探究包膜的变化，为包膜挛缩的治疗提供参考。使用人成纤维细胞（HFF-1）进行实验。采用CCK8法测定积雪草苷和碳硅胶对细胞增殖的联合作用，通过Transwell实验检测细胞迁移变化，用流式细胞术检测细胞周期变化，通过免疫荧光检测成纤维细胞转化标志物（波形蛋白和α-SMA）、胶原蛋白（Col-1A1）以及TGF-β/Smad信号通路相关蛋白（TGF-β1、TβRI、TβRII和Smad2/3）的表达水平。通过将材料皮下植入SD大鼠体内进行实验，并同时口服积雪草苷。采用WB和ELISA检测TGF-β/Smad信号通路相关蛋白表达的变化。然后通过HE、Masson和免疫组织化学染色检测并确认TGF-β/Smad信号通路蛋白。结果显示，积雪草苷联合碳离子植入可抑制硅胶上成纤维细胞的活力、增殖和迁移。免疫荧光显示，α-SMA和Col-1A1的分泌水平显著降低，成纤维细胞向肌成纤维细胞的转化减弱，且TGF-β/Smad信号通路受到抑制。实验结果表明，积雪草苷联合碳硅胶可抑制TGF-β1分泌并抑制TGF-β/Smad信号通路，减少包膜厚度和胶原蛋白沉积。这些结果表明，碳硅胶联合积雪草苷可通过抑制TGF-β/Smad信号通路来调节成纤维细胞的活力、增殖和迁移，并减少包膜厚度和胶原蛋白沉积，从而大大降低包膜挛缩的发生率。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/1a7273f5fb41/fbioe-10-810244-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/ae42e215b2d1/fbioe-10-810244-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/fbbd5b80d860/fbioe-10-810244-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/211745292307/fbioe-10-810244-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/09118d34d602/fbioe-10-810244-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/6ca5f1823408/fbioe-10-810244-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/ea8afff3f230/fbioe-10-810244-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/55a3fc90032f/fbioe-10-810244-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/f4c7f0fdc032/fbioe-10-810244-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/1a7273f5fb41/fbioe-10-810244-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/ae42e215b2d1/fbioe-10-810244-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/fbbd5b80d860/fbioe-10-810244-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/211745292307/fbioe-10-810244-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/09118d34d602/fbioe-10-810244-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/6ca5f1823408/fbioe-10-810244-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/ea8afff3f230/fbioe-10-810244-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/55a3fc90032f/fbioe-10-810244-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/f4c7f0fdc032/fbioe-10-810244-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/dbe3/9133697/1a7273f5fb41/fbioe-10-810244-g009.jpg

相似文献

[1]
Asiaticoside Combined With Carbon Ion Implantation to Improve the Biocompatibility of Silicone Rubber and to Reduce the Risk of Capsule Contracture.

Front Bioeng Biotechnol. 2022-5-12

[2]
Biofunctionalization of silicone rubber with microgroove-patterned surface and carbon-ion implantation to enhance biocompatibility and reduce capsule formation.

Int J Nanomedicine. 2016-10-25

[3]
Effect of Botulinum Toxin Type A on TGF-β/Smad Pathway Signaling: Implications for Silicone-Induced Capsule Formation.

Plast Reconstr Surg. 2016-11

[4]
A multi-targeting bionanomatrix coating to reduce capsular contracture development on silicone implants.

Biomater Res. 2023-4-22

[5]
Asiaticoside suppresses collagen expression and TGF-β/Smad signaling through inducing Smad7 and inhibiting TGF-βRI and TGF-βRII in keloid fibroblasts.

Arch Dermatol Res. 2011-1-15

[6]
Progranulin Promotes the Formation and Development of Capsules Caused by Silicone in Sprague-Dawley Rats.

Clin Cosmet Investig Dermatol. 2022-8-6

[7]
Asiaticoside inhibits TGF-β1-induced mesothelial-mesenchymal transition and oxidative stress via the Nrf2/HO-1 signaling pathway in the human peritoneal mesothelial cell line HMrSV5.

Cell Mol Biol Lett. 2020

[8]
Carbon Ion Implantation: A Good Method to Enhance the Biocompatibility of Silicone Rubber.

Plast Reconstr Surg. 2016-4

[9]
[Interventional effect of asiaticosdide on rats exposed to silica dust].

Zhonghua Lao Dong Wei Sheng Zhi Ye Bing Za Zhi. 2022-1-20

[10]
Platelet-rich plasma attenuates the severity of joint capsule fibrosis following post-traumatic joint contracture in rats.

Front Bioeng Biotechnol. 2023-1-4

引用本文的文献

[1]
Advances in Etiology and Prevention of Capsular Contracture After Breast Implantation.

Aesthetic Plast Surg. 2025-4

本文引用的文献

[1]
Contemporary Breast Augmentation Practice in the United States.

Ann Plast Surg. 2021-3-1

[2]
Breast Augmentation.

Clin Plast Surg. 2021-1

[3]
Review of silicone surface modification techniques and coatings for antibacterial/antimicrobial applications to improve breast implant surfaces.

Acta Biomater. 2021-2

[4]
Short-term Safety of a Silicone Gel-filled Breast Implant: A Manufacturer-sponsored, Retrospective Study.

Plast Reconstr Surg Glob Open. 2020-5-14

[5]
Doxycycline-Coated Silicone Breast Implants Reduce Acute Surgical-Site Infection and Inflammation.

Plast Reconstr Surg. 2020-11

[6]
Dermal fibroblasts have different extracellular matrix profiles induced by TGF-β, PDGF and IL-6 in a model for skin fibrosis.

Sci Rep. 2020-10-14

[7]
Therapeutic Potential of and Its Triterpenes: A Review.

Front Pharmacol. 2020-9-4

[8]
Subclinical Infection of the Silicone Breast Implant Surface as a Possible Cause of Capsular Contracture.

Aesthetic Plast Surg. 2020-8

[9]
Evasion of apoptosis by myofibroblasts: a hallmark of fibrotic diseases.

Nat Rev Rheumatol. 2019-12-2

[10]
Polyurethane-biomacromolecule combined foam dressing containing asiaticoside: fabrication, characterization and clinical efficacy for traumatic dermal wound treatment.

Int J Biol Macromol. 2019-11-25

文献AI研究员

20分钟写一篇综述，助力文献阅读效率提升50倍

立即体验

用中文搜PubMed

大模型驱动的PubMed中文搜索引擎

马上搜索

推荐工具

医学文档翻译智能文献检索