Zhang Yuqing, Song Chunlin, Wang Lei, Jiang Hongfei, Zhai Yujing, Wang Ying, Fang Jing, Zhang Gang
The Cancer Institute, The Affiliated Hospital of Qingdao University, Qingdao, China.
School of Public Health, Qingdao University, Qingdao, China.
Front Cell Dev Biol. 2022 May 13;10:870745. doi: 10.3389/fcell.2022.870745. eCollection 2022.
When eukaryotic cells enter mitosis, dispersed chromosomes move to the cell center along microtubules to form a metaphase plate which facilitates the accurate chromosome segregation. Meanwhile, kinetochores not stably attached by microtubules activate the spindle assembly checkpoint and generate a wait signal to delay the initiation of anaphase. These events are highly coordinated. Disruption of the coordination will cause severe problems like chromosome gain or loss. Bub1, a conserved serine/threonine kinase, plays important roles in mitosis. After extensive studies in the last three decades, the role of Bub1 on checkpoint has achieved a comprehensive understanding; its role on chromosome alignment also starts to emerge. In this review, we summarize the latest development of Bub1 on supporting the two mitotic events. The essentiality of Bub1 in higher eukaryotic cells is also discussed. At the end, some undissolved questions are raised for future study.
当真核细胞进入有丝分裂时，分散的染色体沿着微管移动到细胞中心，形成一个中期板，这有助于准确的染色体分离。同时，未被微管稳定附着的动粒激活纺锤体组装检查点，并产生一个等待信号来延迟后期的启动。这些事件高度协调。协调的破坏会导致严重的问题，如染色体的增加或丢失。Bub1是一种保守的丝氨酸/苏氨酸激酶，在有丝分裂中起重要作用。在过去三十年的广泛研究之后，Bub1在检查点上的作用已经得到了全面的理解；它在染色体排列上的作用也开始显现。在这篇综述中，我们总结了Bub1在支持这两个有丝分裂事件方面的最新进展。还讨论了Bub1在高等真核细胞中的重要性。最后，提出了一些有待解决的问题以供未来研究。
