Turini Luca, Mantino Alberto, Tozzi Beatrice, Bonelli Francesca, Silvi Alina, Mele Marcello, Sgorbini Micaela, Meucci Valentina, Minieri Sara
Dipartimento di Scienze Agrarie, Alimentari, Agro-ambientali, University of Pisa, Pisa, Italy.
Centro di Ricerche Agro-Ambientali "E. Avanzi", University of Pisa, Pisa, Italy.
Front Vet Sci. 2022 May 11;9:873194. doi: 10.3389/fvets.2022.873194. eCollection 2022.
The aims of the present study were to evaluate the preventive and the therapeutic effect of Stodi as phytogenic feed additive rich in phenolic substances on the calf diarrhea, during the first 24 days of life. A total of 40 calves were included and randomly divided into Group C (control group) and Group T (treated group) with placebo or treatment administration started from the third day of life (T0). Calves belonged to group C received 2 L of warm water, while the calves assigned to group T received 2L of warm water plus 30 g of Stodi. Solutions administration was maintained until day 21 (T21) that was the end of the experimental period. Calves were weighed at T0 and T21 to assess the average daily gain (ADG). Physical examination and fecal score evaluation were performed daily. The duration of a diarrheic episode, the age of the first diarrhea outbreak (TDE) and the frequency of diarrheic episodes were recorded. Complete blood count, methemoglobin and liver enzymes were evaluated at T0 and at T21 in all the calves by spectrophotometer and clinical chemistry analysis, respectively. Data were analyzed using a mixed model. A Chi-square and a Mann-Whitney test were also performed. No difference was found for ADG between the groups. The difference of mean age at TDE was not statistically significant between C and T group. The number of calves with diarrhea in the C group tended to be higher than that of T group ( = 0.13). Calves in group C spent more days with clinical sign of diarrhea compared to group T ( = 0.016). Complete blood count, methemoglobin and liver enzymes were within the reference ranges. The feed additive Stodi seemed to be effective in shortening neonatal diarrhea episodes in calves thanks to the administration of 30 g per day of product. The fixed dosage of Stodi used in our study did not show a preventive effect to reduce the incidence of calf diarrhea.
本研究的目的是评估富含酚类物质的植物源饲料添加剂斯托迪（Stodi）在犊牛出生后第1天至第24天对犊牛腹泻的预防和治疗效果。总共纳入40头犊牛，随机分为C组（对照组）和T组（治疗组），从出生第3天（T0）开始给予安慰剂或进行治疗。C组的犊牛饮用2升温水，而分配到T组的犊牛饮用2升温水加30克斯托迪。溶液给药持续至实验期结束的第21天（T21）。在T0和T21对犊牛称重以评估平均日增重（ADG）。每天进行体格检查和粪便评分评估。记录腹泻发作的持续时间、首次腹泻爆发的年龄（TDE）和腹泻发作的频率。在T0和T21分别通过分光光度计和临床化学分析对所有犊牛进行全血细胞计数、高铁血红蛋白和肝酶评估。数据采用混合模型进行分析。还进行了卡方检验和曼-惠特尼检验。两组之间的ADG没有差异。C组和T组之间TDE时的平均年龄差异无统计学意义。C组腹泻犊牛的数量倾向于高于T组（P = 0.13）。与T组相比，C组犊牛出现腹泻临床症状的天数更多（P = 0.016）。全血细胞计数、高铁血红蛋白和肝酶均在参考范围内。由于每天给予30克产品，饲料添加剂斯托迪似乎在缩短犊牛新生儿腹泻发作方面有效。我们研究中使用的斯托迪固定剂量未显示出降低犊牛腹泻发病率的预防作用。
