Si Zengzhi, Qiao Yake, Zhang Kai, Ji Zhixin, Han Jinling
Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science & Technology, Qinghuangdao, 066000, Hebei Province, China.
Open Life Sci. 2022 May 12;17(1):497-511. doi: 10.1515/biol-2022-0052. eCollection 2022.
The most predominant type of resistance (R) genes contain nucleotide-binding sites and leucine-rich repeat (NBS-LRR) domains, characterization of which is helpful for plant resistance improvement. However, the NBS genes of (H.B.K.) remain insufficient to date. In this study, a genome-wide analysis of the NBS-encoding gene in (H.B.K.) was carried out. A total of 442 NBS encoding genes were identified, amounting to 1.37% of the total genes of (H.B.K.). Based on the analysis of the domains, the identified genes were further classified into seven groups: CNL, NL, CN, N, TNL, TN, and RNL. Phylogenetic analysis showed that the NBS genes clustered into three independent clades: RNL, TNL, and CNL. Chromosome location analysis revealed that the distribution of genes in chromosomes was uneven, with a number ranging from 3 to 45. Multiple stress-related regulatory elements were detected in the promoters of the NBS-encoding genes, and their expression profiles were obtained. The qRT-PCR analysis revealed that , , , and 88 responded to stem nematode infection. These results provide critical proof for further characterization and analysis of NBS-encoding genes with important functions.
最主要的抗性（R）基因类型包含核苷酸结合位点和富含亮氨酸重复序列（NBS-LRR）结构域，对其进行表征有助于提高植物抗性。然而，迄今为止，（H.B.K.）的NBS基因仍然不足。在本研究中，对（H.B.K.）中编码NBS的基因进行了全基因组分析。共鉴定出442个编码NBS的基因，占（H.B.K.）总基因数的1.37%。基于结构域分析，将鉴定出的基因进一步分为七组：CNL、NL、CN、N、TNL、TN和RNL。系统发育分析表明，（H.B.K.）的NBS基因聚为三个独立的进化枝：RNL、TNL和CNL。染色体定位分析显示，（H.B.K.）基因在染色体上的分布不均匀，数量从3到45不等。在编码NBS的基因启动子中检测到多个与胁迫相关的调控元件，并获得了它们的表达谱。qRT-PCR分析表明，（基因名称未给出）、（基因名称未给出）、（基因名称未给出）和88个基因对茎线虫感染有响应。这些结果为进一步表征和分析具有重要功能的编码NBS的基因提供了关键证据。
