Wu Jing, Zhu Jifeng, Wang Lanfen, Wang Shumin
Institute of Crop Sciences, Chinese Academy of Agricultural SciencesBeijing, China.
Front Plant Sci. 2017 Aug 9;8:1398. doi: 10.3389/fpls.2017.01398. eCollection 2017.
Nucleotide-binding site and leucine-rich repeat (NBS-LRR) genes represent the largest and most important disease resistance genes in plants. The genome sequence of the common bean ( L.) provides valuable data for determining the genomic organization of NBS-LRR genes. However, data on the NBS-LRR genes in the common bean are limited. In total, 178 NBS-LRR-type genes and 145 partial genes (with or without a NBS) located on 11 common bean chromosomes were identified from genome sequences database. Furthermore, 30 NBS-LRR genes were classified into Toll/interleukin-1 receptor (TIR)-NBS-LRR (TNL) types, and 148 NBS-LRR genes were classified into coiled-coil (CC)-NBS-LRR (CNL) types. Moreover, the phylogenetic tree supported the division of these PvNBS genes into two obvious groups, TNL types and CNL types. We also built expression profiles of NBS genes in response to anthracnose and common bacterial blight using qRT-PCR. Finally, we detected nine disease resistance loci for anthracnose (ANT) and seven for common bacterial blight (CBB) using the developed NBS-SSR markers. Among these loci, NSSR24, NSSR73, and NSSR265 may be located at new regions for ANT resistance, while NSSR65 and NSSR260 may be located at new regions for CBB resistance. Furthermore, we validated NSSR24, NSSR65, NSSR73, NSSR260, and NSSR265 using a new natural population. Our results provide useful information regarding the function of the NBS-LRR proteins and will accelerate the functional genomics and evolutionary studies of NBS-LRR genes in food legumes. NBS-SSR markers represent a wide-reaching resource for molecular breeding in the common bean and other food legumes. Collectively, our results should be of broad interest to bean scientists and breeders.
核苷酸结合位点和富含亮氨酸重复序列（NBS-LRR）基因是植物中最大且最重要的抗病基因。菜豆（Phaseolus vulgaris L.）的基因组序列为确定NBS-LRR基因的基因组组织提供了有价值的数据。然而，关于菜豆中NBS-LRR基因的数据有限。从基因组序列数据库中总共鉴定出位于11条菜豆染色体上的178个NBS-LRR型基因和145个部分基因（有或没有NBS）。此外，30个NBS-LRR基因被分类为Toll/白细胞介素-1受体（TIR）-NBS-LRR（TNL）类型，148个NBS-LRR基因被分类为卷曲螺旋（CC）-NBS-LRR（CNL）类型。而且，系统发育树支持将这些菜豆NBS基因分为两个明显的组，即TNL类型和CNL类型。我们还使用qRT-PCR构建了NBS基因响应炭疽病和普通细菌性疫病的表达谱。最后，我们使用开发的NBS-SSR标记检测到9个炭疽病（ANT）抗病位点和7个普通细菌性疫病（CBB）抗病位点。在这些位点中，NSSR24、NSSR73和NSSR265可能位于ANT抗性的新区域，而NSSR65和NSSR260可能位于CBB抗性的新区域。此外，我们使用一个新的自然群体验证了NSSR24、NSSR65、NSSR73、NSSR260和NSSR265。我们的结果为NBS-LRR蛋白的功能提供了有用信息，并将加速食用豆类中NBS-LRR基因的功能基因组学和进化研究。NBS-SSR标记是菜豆和其他食用豆类分子育种的广泛资源。总体而言，我们的结果应该会引起豆类科学家和育种者的广泛兴趣。
