• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

四个物种中核苷酸结合位点编码基因的全基因组比较分析

Genome-wide comparative analysis of the nucleotide-binding site-encoding genes in four species.

作者信息

Si Zengzhi, Wang Lianjun, Qiao Yake, Roychowdhury Rajib, Ji Zhixin, Zhang Kai, Han Jinling

机构信息

Hebei Key Laboratory of Crop Stress Biology, Hebei Normal University of Science and Technology, Qinhuangdao, China.

Institute of Food Crops, Hubei Academy of Agricultural Sciences, Wuhan, China.

出版信息

Front Plant Sci. 2022 Aug 18;13:960723. doi: 10.3389/fpls.2022.960723. eCollection 2022.

DOI:10.3389/fpls.2022.960723
PMID:36061812
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC9434374/
Abstract

The nucleotide-binding site (NBS)-encoding gene is a major type of resistance (R) gene, and its diverse evolutionary patterns were analyzed in different angiosperm lineages. Until now, no comparative studies have been done on the NBS encoding genes in species. In this study, various numbers of NBS-encoding genes were identified across the whole genome of sweet potato () (#889), (#554), (#571), and (#757). Gene analysis showed that the CN-type and N-type were more common than the other types of NBS-encoding genes. The phylogenetic analysis revealed that the NBS-encoding genes formed three monophyletic clades: CNL, TNL, and RNL, which were distinguished by amino acid motifs. The distribution of the NBS-encoding genes among the chromosomes was non-random and uneven; 83.13, 76.71, 90.37, and 86.39% of the genes occurred in clusters in sweet potato, , , and , respectively. The duplication pattern analysis reveals the presence of higher segmentally duplicated genes in sweet potatoes than tandemly duplicated ones. The opposite trend was found for the other three species. A total of 201 NBS-encoding orthologous genes were found to form synteny gene pairs between any two of the four species, suggesting that each of the synteny gene pairs was derived from a common ancestor. The gene expression patterns were acquired by analyzing using the published datasets. To explore the candidate resistant genes in sweet potato, transcriptome analysis has been carried out using two resistant (JK20 and JK274) and susceptible cultivars (Tengfei and Santiandao) of sweet potato for stem nematodes and pathogen, respectively. A total of 11 differentially expressed genes (DEGs) were found in Tengfei and JK20 for stem nematodes and 19 DEGs in Santiandao and JK274 for . Moreover, six DEGs were further selected for quantitative reverse-transcription polymerase chain reaction (qRT-PCR) analysis, and the results were consistent with the transcriptome analysis. The results may provide new insights into the evolution of NBS-encoding genes in the genome and contribute to the future molecular breeding of sweet potatoes.

摘要

核苷酸结合位点（NBS）编码基因是主要的抗性（R）基因类型，已对其在不同被子植物谱系中的多样进化模式进行了分析。截至目前，尚未对物种中的NBS编码基因开展比较研究。在本研究中，在甘薯（#889）、（#554）、（#571）和（#757）的全基因组中鉴定出了数量各异的NBS编码基因。基因分析表明，CN型和N型比其他类型的NBS编码基因更为常见。系统发育分析显示，NBS编码基因形成了三个单系分支：CNL、TNL和RNL，它们通过氨基酸基序得以区分。NBS编码基因在染色体间的分布并非随机且不均衡；甘薯、、和中分别有83.13%、76.71%、90.37%和86.39%的基因成簇出现。重复模式分析表明，甘薯中片段重复基因的数量高于串联重复基因。在其他三个物种中则发现了相反的趋势。在四个物种中的任意两个之间共发现201个NBS编码直系同源基因形成了同线基因对，这表明每个同线基因对都源自一个共同祖先。通过分析已发表的数据集获取了基因表达模式。为了探究甘薯中的候选抗性基因，分别针对甘薯茎线虫和病原体，利用两个抗性品种（JK20和JK274）以及易感品种（腾飞和三浅道）进行了转录组分析。在腾飞和JK20中针对茎线虫共发现11个差异表达基因（DEG），在三浅道和JK274中针对病原体发现19个DEG。此外，进一步选择了6个DEG进行定量逆转录聚合酶链反应（qRT-PCR）分析，结果与转录组分析一致。这些结果可能为基因组中NBS编码基因的进化提供新见解，并有助于未来甘薯的分子育种。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/9bce787a040d/fpls-13-960723-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f74711b95a14/fpls-13-960723-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/cecfa5cad734/fpls-13-960723-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/5396aa603067/fpls-13-960723-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/38f21d327ebd/fpls-13-960723-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f7fb4ef3cbbf/fpls-13-960723-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f08c6c109da1/fpls-13-960723-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/d171026b3023/fpls-13-960723-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/a2df24faede6/fpls-13-960723-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/9bce787a040d/fpls-13-960723-g009.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f74711b95a14/fpls-13-960723-g001.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/cecfa5cad734/fpls-13-960723-g002.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/5396aa603067/fpls-13-960723-g003.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/38f21d327ebd/fpls-13-960723-g004.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f7fb4ef3cbbf/fpls-13-960723-g005.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/f08c6c109da1/fpls-13-960723-g006.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/d171026b3023/fpls-13-960723-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/a2df24faede6/fpls-13-960723-g008.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/4e02/9434374/9bce787a040d/fpls-13-960723-g009.jpg

相似文献

1
Genome-wide comparative analysis of the nucleotide-binding site-encoding genes in four species.四个物种中核苷酸结合位点编码基因的全基因组比较分析
Front Plant Sci. 2022 Aug 18;13:960723. doi: 10.3389/fpls.2022.960723. eCollection 2022.
2
Genome-Wide Characterization of Nitrogenase Reductase () Genes in the Sweet Potato [ (L.) Lam] and Its Wild Ancestors.甘薯[（L.）Lam]及其野生祖先中固氮酶还原酶（）基因的全基因组特征。
Genes (Basel). 2022 Aug 11;13(8):1428. doi: 10.3390/genes13081428.
3
Genome-wide identification and characterization of NBS-encoding genes in the sweet potato wild ancestor (H.B.K.).甘薯野生祖先（H.B.K.）中NBS编码基因的全基因组鉴定与特征分析。
Open Life Sci. 2022 May 12;17(1):497-511. doi: 10.1515/biol-2022-0052. eCollection 2022.
4
Comparative analysis of the gene family in seven species.七个物种中基因家族的比较分析。
Front Plant Sci. 2023 Mar 20;14:1155018. doi: 10.3389/fpls.2023.1155018. eCollection 2023.
5
Genome-wide comparative analysis of the valine glutamine motif containing genes in four Ipomoea species.对四个番薯属物种中含缬氨酸-谷氨酰胺基序的基因进行全基因组比较分析。
BMC Plant Biol. 2023 Apr 22;23(1):209. doi: 10.1186/s12870-023-04235-6.
6
Diversity and evolutionary relationship of nucleotide binding site-encoding disease-resistance gene analogues in sweet potato (Ipomoea batatas Lam.).甘薯（Ipomoea batatas Lam.）中编码核苷酸结合位点的抗病基因类似物的多样性及进化关系
J Biosci. 2007 Jun;32(4):713-21. doi: 10.1007/s12038-007-0071-7.
7
Genome-wide identification, characterisation and functional evaluation of WRKY genes in the sweet potato wild ancestor Ipomoea trifida (H.B.K.) G. Don. under abiotic stresses.在非生物胁迫下，对甘薯野生祖先Ipomoea trifida（H.B.K.）G. Don 中的 WRKY 基因进行全基因组鉴定、特征分析和功能评估。
BMC Genet. 2019 Dec 3;20(1):90. doi: 10.1186/s12863-019-0789-x.
8
Phylogenetic Analysis of the SQUAMOSA Promoter-Binding Protein-Like Genes in Four Species and Expression Profiling of the During Storage Root Development in Sweet Potato ().甘薯贮藏根发育过程中四个物种中类SQUAMOSA启动子结合蛋白基因的系统发育分析及表达谱分析（）
Front Plant Sci. 2022 Jan 21;12:801061. doi: 10.3389/fpls.2021.801061. eCollection 2021.
9
Genome-Wide Comparative Analysis of the Gene Family in Six Species and the Identification of Anthocyanin-Related Members in Sweet Potatoes.六个物种中基因家族的全基因组比较分析及甘薯中花青素相关成员的鉴定
Plants (Basel). 2023 Apr 21;12(8):1731. doi: 10.3390/plants12081731.
10
Genome-wide comparative analysis of NBS-encoding genes in four Gossypium species.四种棉属植物中NBS编码基因的全基因组比较分析。
BMC Genomics. 2017 Apr 12;18(1):292. doi: 10.1186/s12864-017-3682-x.

引用本文的文献

1
Research Progress in the Mechanisms of Resistance to Biotic Stress in Sweet Potato.甘薯抗生物胁迫机制的研究进展。
Genes (Basel). 2023 Nov 20;14(11):2106. doi: 10.3390/genes14112106.
2
Integrated Transcriptome and Metabolome Analyses Reveal Details of the Molecular Regulation of Resistance to Stem Nematode in Sweet Potato.整合转录组和代谢组分析揭示甘薯抗茎线虫分子调控细节
Plants (Basel). 2023 May 22;12(10):2052. doi: 10.3390/plants12102052.
3
Genome-Wide Comparative Analysis of the Gene Family in Six Species and the Identification of Anthocyanin-Related Members in Sweet Potatoes.

本文引用的文献

1
Genetic Divergence of Lineage-Specific Tandemly Duplicated Gene Clusters in Four Diploid Potato Genotypes.四种二倍体马铃薯基因型中谱系特异性串联重复基因簇的遗传分化
Front Plant Sci. 2022 May 11;13:875202. doi: 10.3389/fpls.2022.875202. eCollection 2022.
2
Phylogenetic Analysis of the SQUAMOSA Promoter-Binding Protein-Like Genes in Four Species and Expression Profiling of the During Storage Root Development in Sweet Potato ().甘薯贮藏根发育过程中四个物种中类SQUAMOSA启动子结合蛋白基因的系统发育分析及表达谱分析（）
Front Plant Sci. 2022 Jan 21;12:801061. doi: 10.3389/fpls.2021.801061. eCollection 2021.
3
六个物种中基因家族的全基因组比较分析及甘薯中花青素相关成员的鉴定
Plants (Basel). 2023 Apr 21;12(8):1731. doi: 10.3390/plants12081731.
4
Genome-Wide Characterization of the PIFs Family in Sweet Potato and Functional Identification of under Drought and Wilt Stresses.甘薯 PIFs 家族的全基因组特征分析及其在干旱和枯萎胁迫下的功能鉴定。
Int J Mol Sci. 2023 Feb 17;24(4):4092. doi: 10.3390/ijms24044092.
An angiosperm NLR Atlas reveals that NLR gene reduction is associated with ecological specialization and signal transduction component deletion.
被子植物 NLR 图谱揭示 NLR 基因减少与生态特化和信号转导元件缺失有关。
Mol Plant. 2021 Dec 6;14(12):2015-2031. doi: 10.1016/j.molp.2021.08.001. Epub 2021 Aug 4.
4
Characterization of Nucleotide Binding -Site-Encoding Genes in Sweetpotato, Ipomoea batatas(L.) Lam., and Their Response to Biotic and Abiotic Stresses.甘薯（Ipomoea batatas(L.) Lam.）核苷酸结合位点编码基因的特征及其对生物和非生物胁迫的响应。
Cytogenet Genome Res. 2021;161(5):257-271. doi: 10.1159/000515834. Epub 2021 Jul 28.
5
Subtelomeric assembly of a multi-gene pathway for antimicrobial defense compounds in cereals.在谷物中，用于抗菌防御化合物的多基因途径的端粒组装。
Nat Commun. 2021 May 7;12(1):2563. doi: 10.1038/s41467-021-22920-8.
6
Distinct Evolutionary Patterns of NBS-Encoding Genes in Three Soapberry Family (Sapindaceae) Species.无患子科三个物种中NBS编码基因的独特进化模式
Front Genet. 2020 Jul 10;11:737. doi: 10.3389/fgene.2020.00737. eCollection 2020.
7
TBtools: An Integrative Toolkit Developed for Interactive Analyses of Big Biological Data.TBtools：一个用于生物大数据交互式分析的集成工具包。
Mol Plant. 2020 Aug 3;13(8):1194-1202. doi: 10.1016/j.molp.2020.06.009. Epub 2020 Jun 23.
8
Key Genes and Genetic Interactions of Plant-Pathogen Functional Modules in Poplar Infected by .杨树感染 后的植物-病原体功能模块的关键基因和遗传相互作用
Mol Plant Microbe Interact. 2020 Aug;33(8):1080-1090. doi: 10.1094/MPMI-11-19-0325-R. Epub 2020 Jun 29.
9
Genome- Wide Analysis of the Nucleotide Binding Site Leucine-Rich Repeat Genes of Four Orchids Revealed Extremely Low Numbers of Disease Resistance Genes.四种兰花核苷酸结合位点富含亮氨酸重复序列基因的全基因组分析显示抗病基因数量极少。
Front Genet. 2020 Jan 8;10:1286. doi: 10.3389/fgene.2019.01286. eCollection 2019.
10
Glycerol-Induced Powdery Mildew Resistance in Wheat by Regulating Plant Fatty Acid Metabolism, Plant Hormones Cross-Talk, and Pathogenesis-Related Genes.甘油通过调节植物脂肪酸代谢、植物激素交叉对话和病程相关基因诱导小麦抗白粉病。
Int J Mol Sci. 2020 Jan 20;21(2):673. doi: 10.3390/ijms21020673.