Centre for Crop Health, University of Southern Queensland, Toowoomba, QLD, Australia.
Loam Bio, Orange, NSW, Australia.
Genome Biol Evol. 2022 May 31;14(6). doi: 10.1093/gbe/evac081.
Charcoal rot is an important soilborne disease caused by a range of Macrophomina species, which affects a broad range of commercially important crops worldwide. Even though Macrophomina species are fungal pathogens of substantial economic importance, their mechanism of pathogenicity and host spectrum are poorly understood. There is an urgent need to better understand the biology, epidemiology, and evolution of Macrophomina species, which, in turn, will aid in improving charcoal rot management strategies. Here, we present the first high-quality genome assembly and annotation of Macrophomina tecta strain BRIP 70781 associated with charcoal rot symptoms on sorghum. Hybrid assembly integrating long reads generated by Oxford Nanopore Technology and short Illumina paired-end reads resulted in 43 contigs with a total assembly size of ∼54 Mb, and an N50 of 3.4 Mb. In total, 12,926 protein-coding genes and 7,036 repeats were predicted. Genome comparisons detected accumulation of DNA transposons in Macrophomina species associated with sorghum. The first reference genome of M. tecta generated in this study will contribute to more comparative and population genomics studies of Macrophomina species.
炭腐病是一种由多种茎点霉属（Macrophomina）引起的重要土传病害，会影响全球范围内广泛的具有商业重要性的作物。尽管茎点霉属真菌病原体具有重要的经济意义，但它们的致病机制和宿主范围仍未得到充分理解。迫切需要更好地了解茎点霉属的生物学、流行病学和进化，这反过来将有助于改进炭腐病管理策略。在这里，我们展示了与高粱炭腐病症状相关的茎点霉属（Macrophomina）BRIP 70781 菌株的第一个高质量基因组组装和注释。整合了 Oxford Nanopore Technology 生成的长读长和短 Illumina 配对末端读长的混合组装得到了 43 个 contigs，总组装大小约为 54 Mb，N50 为 3.4 Mb。总共预测了 12926 个蛋白质编码基因和 7036 个重复序列。基因组比较检测到与高粱相关的茎点霉属中 DNA 转座子的积累。本研究中生成的第一个 M. tecta 参考基因组将有助于茎点霉属的更多比较和群体基因组学研究。
