State Key Laboratory of Food Nutrition and Safety, College of Food Science and Engineering, Tianjin University of Science and Technology, Tianjin 300457, P. R. China.
School of Integrative Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 301617, P. R. China.
Anal Chem. 2022 Jun 14;94(23):8382-8391. doi: 10.1021/acs.analchem.2c00877. Epub 2022 Jun 1.
Bacterial infections seriously threaten human health and also bring huge financial burden. It is critical to construct multifunctional platforms for effectively inactivating bacteria right after point-of-care testing (POCT). Chemiluminescence (CL) bioassays are considered as powerful candidates for POCT as they are free from using an excitation light source, while the flash-type emission limits their further application. Herein, a CL system with long, persistent, and intensive intensity was constructed based on the peroxidase-like property of 4-mercaptophenylboronic acid (MPBA)-functionalized CuSe nanoprobes (CuSe@MPBA), which improved the detection accuracy and sensitivity. By further integrating a smartphone as an analyzer, quantitative POCT of bacteria was realized with high sensitivity. The limit of detection was as low as 1.25 and 1.01 cfu mL for and detection, respectively. Specifically, bacteria can be eliminated with high efficiency due to excellent photothermal property of CuSe@MPBA. The developed multifunctional platform also has advantages of simple operation with low cost, suggesting its high potential for use in food safety, environment monitoring, and clinical applications.
细菌感染严重威胁人类健康，同时也带来了巨大的经济负担。构建多功能平台以在即时检测（POCT）后有效灭活细菌至关重要。化学发光（CL）生物测定法被认为是 POCT 的有力候选者，因为它们无需使用激发光源，而闪烁型发射限制了其进一步的应用。在此，基于 4-巯基苯硼酸（MPBA）功能化 CuSe 纳米探针（CuSe@MPBA）的过氧化物酶样性质，构建了一种具有长寿命、持久和高强度的 CL 系统，提高了检测的准确性和灵敏度。通过进一步将智能手机集成作为分析器，实现了定量 POCT 细菌检测，具有高灵敏度。对于 和 的检测，检测限分别低至 1.25 和 1.01 cfu mL。具体而言，由于 CuSe@MPBA 的优异光热性能，可以高效地消除细菌。开发的多功能平台还具有操作简单、成本低的优点，表明其在食品安全、环境监测和临床应用方面具有很高的应用潜力。
