Hao Zhe, Lin Xiaodong, Li Jinjie, Yin Yanliang, Gao Xia, Wang Shuo, Liu Yaqing
State Key Laboratory of Food Nutrition and Safety, College of Food Engineering and Biotechnology, Tianjin University of Science and Technology, Tianjin, 300457, PR China.
Tianjin Key Laboratory of Food Science and Health, School of Medicine, Nankai University, Tianjin, 300071, PR China.
Biosens Bioelectron. 2021 Feb 1;173:112789. doi: 10.1016/j.bios.2020.112789. Epub 2020 Nov 3.
Bacterial infection is a growing public health concern and causes a huge medical and financial burden. It is of significance to efficiently construct multifunctional platforms for bacterial point-of-care testing (POCT) and elimination. Herein, near-infrared (NIR) light-responded vancomycin-doped prussian blue nanoparticles (PB-VAN) with high efficient photothermal conversion was synthesized for binding, dual-mode portable detection, and elimination of bacteria. The PB-VAN can bind to the surface of Gram-positive bacteria such as Staphylococcus aureus (S. aureus), forming complex of PB-VAN/S. aureus. After being centrifugated, the suspension solution of PB-VAN can stimulate perfluorohexane (PFH) to rapidly release oxygen (O) under NIR irradiation. Thus, the bacteria can be sensitively detected with portable pressure meter as signal reader, reporting a limit of detection (LOD) of 1.0 CFU mL. On the other side, the sediment of PB-VAN/S. aureus can be detected via thermal camera, reporting a LOD of 1.0 CFU mL. Interestingly, the bacteria can be effectively inactivated with the local temperature elevation during temperature-based detection. The antibacterial efficiency reaches as high as 99.8%. The developed multifunctional nanoplatform not only provides a straightforward "mix-then-test" way for portable detection of bacteria with high sensitivity, also realizes high efficiency elimination of bacteria simultaneously. The developed strategy was further applied for promoting wound healing of bacteria-infected mice.
细菌感染日益引起公众对健康的关注，并造成巨大的医疗和经济负担。高效构建用于细菌即时检测（POCT）和消除的多功能平台具有重要意义。在此，合成了具有高效光热转换的近红外（NIR）光响应万古霉素掺杂普鲁士蓝纳米颗粒（PB-VAN），用于结合、双模式便携式检测和消除细菌。PB-VAN可以结合到革兰氏阳性菌如金黄色葡萄球菌（S. aureus）的表面，形成PB-VAN/S. aureus复合物。离心后，PB-VAN悬浮液在近红外照射下可刺激全氟己烷（PFH）快速释放氧气（O）。因此，使用便携式压力计作为信号读取器可以灵敏地检测细菌，检测限（LOD）为1.0 CFU mL。另一方面，PB-VAN/S. aureus沉淀物可通过热成像仪检测，LOD为1.0 CFU mL。有趣的是，在基于温度的检测过程中，细菌可因局部温度升高而被有效灭活。抗菌效率高达99.8%。所开发的多功能纳米平台不仅为高灵敏度的细菌便携式检测提供了一种直接的“混合然后测试”方法，还同时实现了细菌的高效消除。所开发的策略进一步应用于促进细菌感染小鼠的伤口愈合。
