Laboratory of Pharmacology of Pain and Inflammation, Federal University of São João Del Rei, Campus Centro Oeste Dona Lindu, Divinópolis 35501-296, Brazil; Laboratory of Phytochemistry, Federal University of São João Del Rei, Campus Centro Oeste Dona Lindu, Divinópolis 35501-296, Brazil.
Laboratory of Pharmacology of Pain and Inflammation, Federal University of São João Del Rei, Campus Centro Oeste Dona Lindu, Divinópolis 35501-296, Brazil.
Food Res Int. 2022 Jun;156:111145. doi: 10.1016/j.foodres.2022.111145. Epub 2022 Mar 16.
Brazilian biodiversity includes species of the genus Solanum that have several biological activities, in addition to their relevance to agriculture, economics and popular medicine. The ripe fruits of Solanum lycocapum are an important nutritional food source, since they have levels of vitamin C, total soluble sugars, sucrose, phosphorus, and iron comparable or exceed the levels present in fruits such as pineapples, bananas, oranges, and mangoes. The pulp of the fruit is consumed by the population, and it is also used to produce jellies; to make marmalade, replacing the quince, and it can also be mixed with peaches in the preparation of peach. The objective of this study was to evaluate the anti-inflammatory and antioxidant activities of fractions obtained from the ripe fruits and to identify the constituents with these biological properties. The ripe fruits were collected, dried, crushed, and subjected to extraction by exhaustive percolation, obtaining an ethanol extract that was partitioned with solvents of increasing polarities, obtaining hexane (HEX), ethyl acetate (AC), and hydroethanol (HE) fractions. The AC fraction showed higher antioxidant potential compared to BHT (2,6-di-tert-butyl-4-methylphenol) and similar activity to AA (ascorbic acid) by DPPH (1,1-diphenyl-2-picrylhydrazyl) radical assay, while HEX and HE fractions exhibited of IC values similar to BHT. The AC fraction also presented similar activity to BHT by FRAP (Ferric Reducing Antioxidant Power) test. Intraperitoneal treatment with HEX (100 and 300 mg/kg) and HE (100 mg/kg) fractions caused significant inhibition of paw edema induced by carrageenan, 4 and 6 h after the inflammatory stimuli. When analyzed by GC-MS, fatty acids, phytosterols, and triterpenoid were identified in the HEX fraction, whilst 31 compounds were annotated in the AC and HE fractions analyzed by LC-DAD-MS, being phenylpropanoid derivatives, chlorogenic acids, and steroidal glycoalkaloids. The ripe fruits of S. lycocarpum have antioxidant and anti-inflammatory activities, and the detected chemical compounds, especially caffeoylquinic acid derivatives, spermidine, stigmasterol, and β-sitosterol, may be correlated with these activities. The ripe fruits of this species can be a food alternative rich in bioactive compounds and with benefits for human health.
巴西的生物多样性包括茄属植物的物种，这些物种具有多种生物活性，除了在农业、经济和民间医学方面具有重要意义外。成熟的刺茄果实是一种重要的营养食品来源，因为它们的维生素 C、总可溶性糖、蔗糖、磷和铁含量与菠萝、香蕉、橙子和芒果等水果相当或更高。果肉被人们食用，也用于制作果冻；制作果酱，代替榅桲，也可以与桃子混合制作桃子。本研究的目的是评估成熟果实中获得的馏分的抗炎和抗氧化活性，并鉴定具有这些生物特性的成分。成熟的果实被收集、干燥、粉碎，并通过彻底渗滤进行提取，得到乙醇提取物，然后用溶剂进行分配，极性逐渐增加，得到正己烷（HEX）、乙酸乙酯（AC）和水乙醇（HE）馏分。与 BHT（2,6-二叔丁基-4-甲基苯酚）相比，AC 馏分显示出更高的抗氧化潜力，并且通过 DPPH（1,1-二苯基-2-苦基肼）自由基测定法，其活性与 AA（抗坏血酸）相似，而 HEX 和 HE 馏分的 IC 值与 BHT 相似。AC 馏分在 FRAP（铁还原抗氧化能力）试验中也表现出与 BHT 相似的活性。腹腔内给予 HEX（100 和 300mg/kg）和 HE（100mg/kg）馏分可显著抑制角叉菜胶诱导的爪肿胀，在炎症刺激后 4 和 6 小时。当通过 GC-MS 分析时，在 HEX 馏分中鉴定出脂肪酸、植物甾醇和三萜，而在通过 LC-DAD-MS 分析的 AC 和 HE 馏分中注释了 31 种化合物，这些化合物为苯丙素衍生物、绿原酸和甾体糖苷生物碱。S. lycocarpum 成熟果实具有抗氧化和抗炎活性，检测到的化学物质，特别是咖啡酰奎宁酸衍生物、亚精胺、豆甾醇和β-谷甾醇，可能与这些活性有关。该物种的成熟果实可以作为富含生物活性化合物的替代食品，对人类健康有益。
